Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PP479_RS26950 Genome accession   NZ_CP116935
Coordinates   5707154..5707618 (-) Length   154 a.a.
NCBI ID   WP_057386486.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CUVET18-860     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5702154..5712618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PP479_RS26930 (PP479_26920) nadC 5704654..5705502 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PP479_RS26940 (PP479_26930) - 5705683..5706549 (-) 867 WP_225007570.1 O-antigen ligase family protein -
  PP479_RS26945 (PP479_26935) - 5706559..5707065 (-) 507 WP_225007572.1 hypothetical protein -
  PP479_RS26950 (PP479_26940) pilA/pilAI 5707154..5707618 (-) 465 WP_057386486.1 pilin Machinery gene
  PP479_RS26955 (PP479_26945) pilB 5707849..5709548 (+) 1700 Protein_5337 type IV-A pilus assembly ATPase PilB -
  PP479_RS26960 (PP479_26950) pilC 5709552..5710769 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  PP479_RS26965 (PP479_26955) pilD 5710770..5711642 (+) 873 WP_034037193.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PP479_RS26970 (PP479_26960) coaE 5711639..5712250 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PP479_RS26975 (PP479_26965) yacG 5712247..5712447 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16220.53 Da        Isoelectric Point: 8.4551

>NTDB_id=714845 PP479_RS26950 WP_057386486.1 5707154..5707618(-) (pilA/pilAI) [Pseudomonas aeruginosa strain CUVET18-860]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKEQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=714845 PP479_RS26950 WP_057386486.1 5707154..5707618(-) (pilA/pilAI) [Pseudomonas aeruginosa strain CUVET18-860]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGGAGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.132

98.701

0.396
  pilA Vibrio cholerae strain A1552

40.132

98.701

0.396
  pilA Vibrio cholerae C6706

40.132

98.701

0.396
  pilA2 Legionella pneumophila strain ERS1305867

39.189

96.104

0.377
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364