Detailed information NTG0714827

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: NQZ93_RS00775
• Genome accession: NZ_CP102141
• Coordinates: 121741..122175 (+)
• Length: 144 a.a.
• NCBI ID: WP_024382121.1
• Uniprot ID: A0A075SNP0
• Organism: Streptococcus suis strain DNR48
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 116741..127175

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NQZ93_RS00740 (NQZ93_00740)	-	117483..117848 (+)	366	WP_002936568.1	DUF1033 family protein	-
NQZ93_RS00745 (NQZ93_00745)	-	117883..118833 (-)	951	WP_257115308.1	S66 peptidase family protein	-
NQZ93_RS00750 (NQZ93_00750)	comYA	118919..119869 (+)	951	WP_024382125.1	competence type IV pilus ATPase ComGA	Machinery gene
NQZ93_RS00755 (NQZ93_00755)	comYB	119781..120818 (+)	1038	WP_255195823.1	competence type IV pilus assembly protein ComGB	Machinery gene
NQZ93_RS00760 (NQZ93_00760)	comYC	120820..121101 (+)	282	WP_024382124.1	competence type IV pilus major pilin ComGC	Machinery gene
NQZ93_RS00765 (NQZ93_00765)	comGD	121082..121489 (+)	408	WP_024382123.1	competence type IV pilus minor pilin ComGD	-
NQZ93_RS00770 (NQZ93_00770)	comYE	121461..121754 (+)	294	WP_024382122.1	competence type IV pilus minor pilin ComGE	Machinery gene
NQZ93_RS00775 (NQZ93_00775)	comGF/cglF	121741..122175 (+)	435	WP_024382121.1	competence type IV pilus minor pilin ComGF	Machinery gene
NQZ93_RS00780 (NQZ93_00780)	comGG	122153..122563 (+)	411	WP_024382120.1	competence type IV pilus minor pilin ComGG	-
NQZ93_RS00785 (NQZ93_00785)	comYH	122620..123573 (+)	954	WP_257115309.1	class I SAM-dependent methyltransferase	Machinery gene
NQZ93_RS00790 (NQZ93_00790)	-	123623..124810 (+)	1188	WP_024382118.1	acetate kinase	-
NQZ93_RS00795 (NQZ93_00795)	-	125125..125676 (+)	552	WP_024382117.1	folate family ECF transporter S component	-
NQZ93_RS00800 (NQZ93_00800)	-	125732..126988 (+)	1257	WP_024390768.1	folylpolyglutamate synthase/dihydrofolate synthase family protein	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16461.82 Da
• Isoelectric Point: 6.9832

>NTDB_id=714827 NQZ93_RS00775 WP_024382121.1 121741..122175(+) (comGF/cglF) [Streptococcus suis strain DNR48]
MLKSKIPAFTLLECLVALIILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQAELVKVENGKVYVNKGE
QALVFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide

• Length: 435 bp

>NTDB_id=714827 NQZ93_RS00775 WP_024382121.1 121741..122175(+) (comGF/cglF) [Streptococcus suis strain DNR48]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTACCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTCT
CTAGTCAATTGCGGTCGGAGTGGGACCAGGCTGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGGCGAA
CAGGCTTTGGTCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGTTACCAGCCTATGCT
CTATCAGGTGGATAGCGCAGCCATTTCCCAAGAGAACCAGCTGGTGCGCATCGACTTCAGCTTTAAAAACGGAGAGGAGC
GAACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

3D structure

• AlphaFold DB: A0A075SNP0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae TIGR4	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae Rx1	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae D39	53.191	97.917	0.521
comGF/cglF	Streptococcus pneumoniae R6	53.191	97.917	0.521
comGF/cglF	Streptococcus mitis NCTC 12261	53.731	93.056	0.5
comYF	Streptococcus mutans UA140	49.306	100	0.493
comYF	Streptococcus mutans UA159	48.611	100	0.486
comGF/cglF	Streptococcus mitis SK321	51.493	93.056	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	46.809	97.917	0.458