Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   NL422_RS02530 Genome accession   NZ_CP116931
Coordinates   420729..421967 (+) Length   412 a.a.
NCBI ID   WP_019842344.1    Uniprot ID   -
Organism   Escherichia coli strain MLI106K1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415729..426967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NL422_RS02505 (NL422_002505) mrcA 416009..418561 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  NL422_RS02510 (NL422_002510) hofM 418681..419460 (+) 780 WP_001551759.1 DNA utilization protein HofM -
  NL422_RS02515 (NL422_002515) hofN 419460..419999 (+) 540 WP_001069326.1 DNA utilization protein HofN -
  NL422_RS02520 (NL422_002520) hofO 419983..420423 (+) 441 WP_001551756.1 DNA utilization protein HofO -
  NL422_RS02525 (NL422_002525) hofP 420413..420817 (+) 405 WP_001551755.1 DNA utilization protein HofP -
  NL422_RS02530 (NL422_002530) comE 420729..421967 (+) 1239 WP_019842344.1 DNA uptake porin HofQ Machinery gene
  NL422_RS02535 (NL422_002535) aroK 422369..422890 (+) 522 WP_000818618.1 shikimate kinase AroK -
  NL422_RS02540 (NL422_002540) aroB 422947..424035 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  NL422_RS02545 (NL422_002545) damX 424127..425413 (+) 1287 WP_001551753.1 cell division protein DamX -
  NL422_RS02550 (NL422_002550) dam 425520..426356 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44694.18 Da        Isoelectric Point: 5.9488

>NTDB_id=714511 NL422_RS02530 WP_019842344.1 420729..421967(+) (comE) [Escherichia coli strain MLI106K1]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=714511 NL422_RS02530 WP_019842344.1 420729..421967(+) (comE) [Escherichia coli strain MLI106K1]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACGCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCGCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGTTGGCTGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATGTCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCTCATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTTACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGTTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio campbellii strain DS40M4

38.242

100

0.391
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364
  pilQ Pseudomonas aeruginosa PAK

34.174

100

0.362