Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   NQD66_RS01085 Genome accession   NZ_CP101995
Coordinates   187940..188983 (+) Length   347 a.a.
NCBI ID   WP_223295592.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain BCJB2983     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 182940..193983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQD66_RS01075 (NQD66_01075) - 186537..186902 (+) 366 WP_000285373.1 DUF1033 family protein -
  NQD66_RS01080 (NQD66_01080) comYA 187075..188046 (+) 972 WP_000250808.1 competence type IV pilus ATPase ComGA Machinery gene
  NQD66_RS01085 (NQD66_01085) comYB 187940..188983 (+) 1044 WP_223295592.1 competence type IV pilus assembly protein ComGB Machinery gene
  NQD66_RS01090 (NQD66_01090) comYD 188980..189408 (+) 429 WP_000793381.1 competence type IV pilus minor pilin ComGD Machinery gene
  NQD66_RS01095 (NQD66_01095) comGE 189380..189679 (+) 300 WP_001867089.1 competence type IV pilus minor pilin ComGE -
  NQD66_RS01100 (NQD66_01100) comGF 189633..190094 (+) 462 WP_001874060.1 competence type IV pilus minor pilin ComGF -
  NQD66_RS01105 (NQD66_01105) comGG 190072..190443 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  NQD66_RS01110 (NQD66_01110) comYH 190558..191532 (+) 975 WP_001008570.1 class I SAM-dependent methyltransferase Machinery gene
  NQD66_RS01115 (NQD66_01115) - 191564..192757 (+) 1194 WP_000047535.1 acetate kinase -
  NQD66_RS01120 (NQD66_01120) - 192908..193114 (+) 207 WP_000798242.1 helix-turn-helix transcriptional regulator -
  NQD66_RS01125 (NQD66_01125) - 193173..193310 (+) 138 WP_001867090.1 hypothetical protein -
  NQD66_RS01130 (NQD66_01130) - 193351..193806 (+) 456 WP_000905674.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 40005.70 Da        Isoelectric Point: 9.9365

>NTDB_id=713944 NQD66_RS01085 WP_223295592.1 187940..188983(+) (comYB) [Streptococcus agalactiae strain BCJB2983]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDYYTDRMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLTFLVLIMIGLRHYLMPQLGENNFATS
LITNVPNIFLLLLAVVLIFSLIFYIVQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=713944 NQD66_RS01085 WP_223295592.1 187940..188983(+) (comYB) [Streptococcus agalactiae strain BCJB2983]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTATTATACAGATAGGATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGACTTTTCTGGTGCTAATTATGATTGGTCTTAGGCATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGC
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTGTTCA
AAAGCGATTGTCGCGTATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTGTTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCTGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

61.877

98.271

0.608
  comYB Streptococcus mutans UA159

61.584

98.271

0.605
  comYB Streptococcus gordonii str. Challis substr. CH1

56.305

98.271

0.553
  comGB/cglB Streptococcus mitis NCTC 12261

54.925

96.542

0.53
  comGB/cglB Streptococcus mitis SK321

54.328

96.542

0.524
  comGB/cglB Streptococcus pneumoniae Rx1

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae D39

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae R6

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

53.433

96.542

0.516
  comGB Lactococcus lactis subsp. cremoris KW2

49.852

97.118

0.484