Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   CHBNTHi_RS03360 Genome accession   NZ_AP018781
Coordinates   664415..665752 (+) Length   445 a.a.
NCBI ID   WP_110437669.1    Uniprot ID   -
Organism   Haemophilus influenzae strain CHBN-V-1 isolate 13H43     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 659415..670752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHBNTHi_RS03335 (CHBNV1_06310) - 659480..662074 (-) 2595 WP_110437668.1 penicillin-binding protein 1A -
  CHBNTHi_RS03340 (CHBNV1_06320) comA 662173..662970 (+) 798 WP_011272050.1 pilus assembly protein PilM Machinery gene
  CHBNTHi_RS03345 (CHBNV1_06330) comB 662971..663477 (+) 507 WP_011272049.1 competence protein ComB Machinery gene
  CHBNTHi_RS03350 (CHBNV1_06340) comC 663474..663995 (+) 522 WP_011272048.1 competence protein ComC Machinery gene
  CHBNTHi_RS03355 (CHBNV1_06350) comD 663992..664405 (+) 414 WP_011272047.1 pilus assembly protein PilP Machinery gene
  CHBNTHi_RS03360 (CHBNV1_06360) comE 664415..665752 (+) 1338 WP_110437669.1 type IV pilus secretin PilQ Machinery gene
  CHBNTHi_RS03365 (CHBNV1_06370) - 665765..666451 (+) 687 WP_110437670.1 ComF family protein -
  CHBNTHi_RS03370 (CHBNV1_06380) nfuA 666527..667123 (+) 597 WP_005649300.1 Fe-S biogenesis protein NfuA -
  CHBNTHi_RS03375 (CHBNV1_06390) nudC 667190..667984 (+) 795 WP_110437671.1 NAD(+) diphosphatase -
  CHBNTHi_RS03380 (CHBNV1_06400) - 668020..668610 (+) 591 WP_110437672.1 YjaG family protein -
  CHBNTHi_RS03385 (CHBNV1_06410) - 668751..669023 (+) 273 WP_005630954.1 HU family DNA-binding protein -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49079.40 Da        Isoelectric Point: 8.1445

>NTDB_id=71317 CHBNTHi_RS03360 WP_110437669.1 664415..665752(+) (comE) [Haemophilus influenzae strain CHBN-V-1 isolate 13H43]
MKKYFLKCGYFLVCFCLPLIVFANPKTDNECFFIRLSQAPLAQTLEQLAFQQDVNLVMGERLEGNISLKLNNIDMPRLLK
IIAKSKHLTLNKDDGVYYLNGSQSGKGQVAGNLTTNEPHLVSHTVKLHFAKASELMKSLTTGSGSLLSSAGSITFDDRSN
LLVIQDEPRFVQNIKKLIAEMDKPIEQIAIEARIVTITDESLKELGVRWGIFNPTENARRVAGSLTGNSFENIADNLNVN
FATTTTPAGSIALQVAKINGRLLDLELSALERENNVEIIASPRLLTTNKKSASIKQGTEIPYIVSNTRNDTQSVEFREAV
LGLEVTPHISKDNNILLDLLVSQNSPGSRVAYGQNEVVSIDKQEINTQVFAKDGETIVLGGVFHDTITKSEDKVPLLGDI
PVIKRLFSKESERHQKRELVIFVTPHILKAGETLAALKQKSAGKK

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=71317 CHBNTHi_RS03360 WP_110437669.1 664415..665752(+) (comE) [Haemophilus influenzae strain CHBN-V-1 isolate 13H43]
ATGAAGAAATATTTTTTAAAGTGCGGTTATTTTTTAGTGTGTTTTTGTTTGCCATTAATCGTTTTTGCTAATCCTAAAAC
AGATAACGAATGTTTTTTTATTCGTTTATCGCAAGCACCTTTAGCTCAAACACTGGAGCAATTAGCTTTTCAACAAGATG
TGAATTTAGTGATGGGTGAGAGGTTAGAAGGCAATATTTCTTTGAAATTAAACAATATTGATATGCCACGTTTGCTAAAA
ATAATCGCAAAAAGTAAGCATCTTACTTTGAATAAAGATGATGGGGTTTATTATTTAAACGGCAGTCAATCTGGCAAAGG
TCAAGTTGCAGGAAATCTTACGACAAATGAACCGCACTTAGTCAGCCACACGGTAAAACTTCATTTTGCTAAAGCCTCTG
AATTAATGAAATCCTTAACAACAGGAAGTGGATCTTTGCTTTCTTCTGCGGGGAGCATTACCTTTGATGATCGCAGTAAT
TTGCTGGTTATTCAGGATGAACCTCGTTTTGTGCAAAATATCAAAAAACTGATTGCTGAAATGGATAAGCCTATTGAACA
GATCGCTATTGAAGCGCGAATTGTGACAATTACGGATGAGAGTTTGAAAGAACTTGGCGTTCGGTGGGGGATTTTTAATC
CAACTGAAAATGCAAGACGAGTTGCGGGCAGCCTTACAGGCAATAGCTTTGAAAATATTGCGGATAATCTTAATGTAAAT
TTTGCGACAACGACGACACCTGCTGGCTCTATAGCATTACAAGTCGCGAAAATTAATGGGCGATTGCTTGATTTAGAATT
GAGTGCGTTGGAGCGTGAAAATAATGTAGAAATTATTGCAAGTCCTCGCTTACTCACTACCAATAAGAAAAGTGCGAGCA
TTAAACAGGGGACAGAAATTCCTTACATCGTGAGTAATACTCGTAACGATACGCAATCTGTGGAATTTCGTGAGGCGGTG
CTTGGTTTGGAAGTGACGCCACATATCTCTAAAGATAACAATATCTTACTTGATTTATTGGTAAGTCAAAATTCCCCTGG
TTCTCGTGTCGCTTATGGACAAAATGAGGTGGTTTCTATTGATAAACAAGAAATTAATACTCAGGTTTTTGCCAAAGATG
GGGAAACCATTGTGCTTGGCGGCGTGTTCCACGATACGATCACGAAAAGCGAAGATAAAGTGCCATTGCTTGGCGATATA
CCCGTTATTAAACGATTATTTAGCAAAGAAAGTGAACGACATCAAAAACGTGAGCTAGTGATTTTCGTCACGCCACATAT
TTTAAAAGCAGGAGAAACGTTAGCGGCGTTGAAACAAAAAAGTGCGGGGAAAAAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(123-187)

Bacterial type II and III secretion system protein (Secretin)

(269-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

99.775

100

0.998
  comE Haemophilus influenzae Rd KW20

97.303

100

0.973
  comE Glaesserella parasuis strain SC1401

52

95.506

0.497
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.625

100

0.409
  pilQ Vibrio cholerae strain A1552

40.625

100

0.409
  pilQ Vibrio campbellii strain DS40M4

42.959

94.157

0.404

Multiple sequence alignment