Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   NOV99_RS10170 Genome accession   NZ_CP101844
Coordinates   2077051..2077485 (-) Length   144 a.a.
NCBI ID   WP_002936547.1    Uniprot ID   A0A0Z8H0U1
Organism   Streptococcus suis strain 2018WUSS151     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2072051..2082485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOV99_RS10145 (NOV99_10145) - 2072240..2073496 (-) 1257 WP_002936539.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -
  NOV99_RS10150 (NOV99_10150) - 2073550..2074101 (-) 552 WP_014636756.1 folate family ECF transporter S component -
  NOV99_RS10155 (NOV99_10155) - 2074416..2075603 (-) 1188 WP_002936543.1 acetate kinase -
  NOV99_RS10160 (NOV99_10160) comYH 2075653..2076606 (-) 954 WP_002936545.1 class I SAM-dependent methyltransferase Machinery gene
  NOV99_RS10165 (NOV99_10165) comGG 2076663..2077073 (-) 411 WP_002936546.1 competence type IV pilus minor pilin ComGG -
  NOV99_RS10170 (NOV99_10170) comGF/cglF 2077051..2077485 (-) 435 WP_002936547.1 competence type IV pilus minor pilin ComGF Machinery gene
  NOV99_RS10175 (NOV99_10175) comYE 2077472..2077765 (-) 294 WP_002936548.1 competence type IV pilus minor pilin ComGE Machinery gene
  NOV99_RS10180 (NOV99_10180) comGD 2077737..2078144 (-) 408 WP_014636753.1 competence type IV pilus minor pilin ComGD -
  NOV99_RS10185 (NOV99_10185) comYC 2078125..2078406 (-) 282 WP_002936550.1 competence type IV pilus major pilin ComGC Machinery gene
  NOV99_RS10190 (NOV99_10190) comYB 2078408..2079445 (-) 1038 WP_255195823.1 competence type IV pilus assembly protein ComGB Machinery gene
  NOV99_RS10195 (NOV99_10195) comYA 2079357..2080307 (-) 951 WP_002936562.1 competence type IV pilus ATPase ComGA Machinery gene
  NOV99_RS10200 (NOV99_10200) - 2080393..2081343 (+) 951 WP_002936565.1 S66 peptidase family protein -
  NOV99_RS10205 (NOV99_10205) - 2081378..2081743 (-) 366 WP_002936568.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16580.00 Da        Isoelectric Point: 6.9819

>NTDB_id=712332 NOV99_RS10170 WP_002936547.1 2077051..2077485(-) (comGF/cglF) [Streptococcus suis strain 2018WUSS151]
MLKSKIPAFTLLECLVALIILSGSLLVFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRLEWDQSDLVKVENGKVYVNKVE
QALAFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAIYQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=712332 NOV99_RS10170 WP_002936547.1 2077051..2077485(-) (comGF/cglF) [Streptococcus suis strain 2018WUSS151]
TTGTTAAAAAGTAAAATTCCTGCTTTTACTCTCTTGGAATGCTTGGTAGCTTTAATCATCCTATCAGGAAGTCTTCTGGT
CTTTGAAGGCTTGTCAAAACTGCTGGTCCAAGAAGCGCATTATCAAGGTCAGACCGTTCAAAAGGAATGGTTGGTCTTTT
CTAGCCAGTTGCGGTTGGAGTGGGACCAGTCGGACTTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAACAAAGTCGAA
CAGGCTTTGGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACAAATGACAGGGGACAGGGTTACCAGCCCATGCT
CTATCAGGTGGATAGCGCAGCCATTTACCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATTTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Z8H0U1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

54.61

97.917

0.535
  comGF/cglF Streptococcus pneumoniae D39

54.61

97.917

0.535
  comGF/cglF Streptococcus pneumoniae R6

54.61

97.917

0.535
  comGF/cglF Streptococcus pneumoniae TIGR4

54.61

97.917

0.535
  comGF/cglF Streptococcus mitis NCTC 12261

55.224

93.056

0.514
  comGF/cglF Streptococcus mitis SK321

52.239

93.056

0.486
  comYF Streptococcus mutans UA140

48.611

100

0.486
  comYF Streptococcus mutans UA159

47.917

100

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

45.39

97.917

0.444