Detailed information NTG0711091

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: POF62_RS00410
• Genome accession: NZ_CP116707
• Coordinates: 64942..65940 (+)
• Length: 332 a.a.
• NCBI ID: WP_000196633.1
• Uniprot ID: Q3K3X8
• Organism: Streptococcus agalactiae strain A909 sCas9revertant
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 59942..70940

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF62_RS00395 (POF62_00395)	-	60969..62360 (+)	1392	WP_001079797.1	hypothetical protein	-
POF62_RS00400 (POF62_00400)	purB	62382..63680 (+)	1299	WP_000572890.1	adenylosuccinate lyase	-
POF62_RS00405 (POF62_00405)	comR	63833..64741 (+)	909	WP_000912095.1	XRE family transcriptional regulator	Regulator
POF62_RS00410 (POF62_00410)	ruvB	64942..65940 (+)	999	WP_000196633.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
POF62_RS00415 (POF62_00415)	-	66092..66529 (+)	438	WP_000754818.1	low molecular weight protein-tyrosine-phosphatase	-
POF62_RS00420 (POF62_00420)	-	66536..66916 (+)	381	WP_000787702.1	membrane protein	-
POF62_RS00425 (POF62_00425)	-	66913..68691 (+)	1779	WP_001220907.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37573.90 Da
• Isoelectric Point: 4.5053

>NTDB_id=711091 POF62_RS00410 WP_000196633.1 64942..65940(+) (ruvB) [Streptococcus agalactiae strain A909 sCas9revertant]
MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAIEKSGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPRIANRLLKRVRDYAQIMGDGLID
DNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATDK
AYEHLGYQRFDK

Nucleotide

• Length: 999 bp

>NTDB_id=711091 POF62_RS00410 WP_000196633.1 64942..65940(+) (ruvB) [Streptococcus agalactiae strain A909 sCas9revertant]
ATGACAAGATTTTTAGATAGTGATGCAATGGGTGACGAAGAATTGGTAGAACGTACACTTCGTCCGCAGTATTTAAGAGA
GTATATTGGACAAGATAAGGTTAAAGATCAGCTAAAAATATTTATTGAAGCTGCTAAATTGCGTGATGAGTCATTGGATC
ATGTGTTATTATTTGGCCCTCCTGGTTTAGGGAAAACAACCATGGCATTTGTAATTGCTAATGAGTTGGGTGTCAATCTC
AAACAAACATCAGGTCCCGCAATTGAAAAATCAGGGGATTTAGTAGCCATTTTAAATGATTTAGAACCAGGTGATGTTCT
TTTTATTGATGAAATTCATCGTATGCCGATGGCGGTTGAAGAGGTACTTTATAGTGCAATGGAAGACTTTTATATTGACA
TTATGATCGGTGCAGGAGAAACTAGTAGAAGTGTTCATTTAGATTTGCCGCCCTTTACCTTAATTGGTGCAACGACACGT
GCAGGTATGTTATCTAATCCCTTACGTGCTCGCTTTGGTATTACAGGGCATATGGAGTATTATGAAGAAAACGATTTGAC
AGAAATTATTGAGCGTACAGCAGATATTTTTGAAATGAAAATTACTTATGAAGCTGCTTCTGAATTAGCGCGTCGCAGTC
GTGGAACGCCACGTATCGCTAACCGTTTATTGAAACGTGTTCGAGATTATGCTCAAATCATGGGAGATGGTTTGATAGAT
GACAATATTACAGATAAAGCATTAACGATGTTAGATGTTGATCACGAGGGGCTTGATTACGTCGATCAAAAAATCTTAAG
AACCATGATTGAAATGTATAATGGAGGTCCTGTTGGTTTAGGAACTCTATCCGTTAATATTGCTGAAGAACGAGATACTG
TTGAAGACATGTACGAACCTTATTTAATTCAAAAAGGTTTTATTATGCGTACCCGTACCGGTCGTGTAGCTACGGATAAG
GCATACGAACATTTAGGTTATCAGCGATTTGATAAATAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

3D structure

• AlphaFold DB: Q3K3X8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae TIGR4	90.06	100	0.901
ruvB	Streptococcus pneumoniae R6	89.759	100	0.898
ruvB	Streptococcus pneumoniae D39	89.759	100	0.898
ruvB	Bacillus subtilis subsp. subtilis str. 168	59.819	99.699	0.596
ruvB	Helicobacter pylori 26695	53.074	93.072	0.494
ruvB	Synechocystis sp. PCC 6803	50.479	94.277	0.476