Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DWB40_RS04110 Genome accession   NZ_CP101622
Coordinates   932480..934213 (+) Length   577 a.a.
NCBI ID   WP_062605785.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain CYZ     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 927480..939213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DWB40_RS04080 - 927511..927897 (+) 387 WP_231336626.1 hypothetical protein -
  DWB40_RS04085 coaE 928157..928768 (-) 612 WP_062605778.1 dephospho-CoA kinase -
  DWB40_RS04090 - 928780..929643 (-) 864 WP_005410800.1 A24 family peptidase -
  DWB40_RS04095 pilC 929651..930910 (-) 1260 WP_062605780.1 type II secretion system F family protein Machinery gene
  DWB40_RS04100 - 931263..931697 (+) 435 WP_062605782.1 pilin -
  DWB40_RS04105 - 931850..932257 (+) 408 WP_062605784.1 pilin -
  DWB40_RS04110 pilB 932480..934213 (+) 1734 WP_062605785.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DWB40_RS04115 pilR 934339..935712 (-) 1374 WP_062605786.1 sigma-54 dependent transcriptional regulator Regulator
  DWB40_RS04120 - 935773..937386 (-) 1614 WP_062605787.1 ATP-binding protein -
  DWB40_RS04125 sucC 937744..938913 (+) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62345.27 Da        Isoelectric Point: 5.8722

>NTDB_id=711060 DWB40_RS04110 WP_062605785.1 932480..934213(+) (pilB) [Stenotrophomonas maltophilia strain CYZ]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGRLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=711060 DWB40_RS04110 WP_062605785.1 932480..934213(+) (pilB) [Stenotrophomonas maltophilia strain CYZ]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGATGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCGGCCGCCCGCCAGCCACTGCCAACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAGGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAACCGATCCTGG
TTGACGAGGACCAGATCCGCCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGACGCCCTGGGGGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCGGCGATTCCGGCATCGACGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGTGCCTCGGACATCC
ACTTCGAGCCCTACGAAGATGATTACCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCACCGGTG
AAGTTGAACCAGCGCATCGCCGCCCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAACCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGATCGGGCAAGACGGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGTCGCCTGTGCGGCAACTGCAAGCGACCGTCCAGCCTGCCCGAA
CATGCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCGTTGAAGAAG
GCCGCGGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.872

100

0.556
  pilB Acinetobacter baumannii D1279779

55

100

0.553
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilF Neisseria gonorrhoeae MS11

48.596

98.787

0.48
  pilB Vibrio cholerae strain A1552

48.677

98.267

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.133

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447