Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NMH04_RS17010 Genome accession   NZ_CP101609
Coordinates   3273564..3274691 (-) Length   375 a.a.
NCBI ID   WP_255816785.1    Uniprot ID   -
Organism   Bacillus altitudinis strain 47     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3268564..3279691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMH04_RS16975 (NMH04_16910) flgL 3268747..3269658 (-) 912 WP_008348257.1 flagellar hook-associated protein FlgL -
  NMH04_RS16980 (NMH04_16915) flgK 3269667..3271187 (-) 1521 WP_024719239.1 flagellar hook-associated protein FlgK -
  NMH04_RS16985 (NMH04_16920) - 3271203..3271685 (-) 483 WP_007499977.1 flagellar protein FlgN -
  NMH04_RS16990 (NMH04_16925) flgM 3271704..3271967 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  NMH04_RS16995 (NMH04_16930) - 3272047..3272466 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  NMH04_RS17000 (NMH04_16935) comFC 3272523..3273209 (-) 687 WP_176544677.1 ComF family protein Machinery gene
  NMH04_RS17005 (NMH04_16940) - 3273206..3273499 (-) 294 WP_008348267.1 late competence development ComFB family protein -
  NMH04_RS17010 (NMH04_16945) comFA 3273564..3274691 (-) 1128 WP_255816785.1 DEAD/DEAH box helicase Machinery gene
  NMH04_RS17015 (NMH04_16950) - 3275063..3275905 (-) 843 WP_024719242.1 DegV family protein -
  NMH04_RS17020 (NMH04_16955) degU 3276117..3276806 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NMH04_RS17025 (NMH04_16960) degS 3276871..3278046 (-) 1176 WP_024719243.1 sensor histidine kinase Regulator
  NMH04_RS17030 (NMH04_16965) - 3278268..3278936 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42976.04 Da        Isoelectric Point: 10.4855

>NTDB_id=710814 NMH04_RS17010 WP_255816785.1 3273564..3274691(-) (comFA) [Bacillus altitudinis strain 47]
MLGKVSECGFLYEWTGPQKKETRRVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGLNIAALYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARRQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQVQDVNVQGVSADDQERKQKVQYFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
SESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=710814 NMH04_RS17010 WP_255816785.1 3273564..3274691(-) (comFA) [Bacillus altitudinis strain 47]
ATGCTGGGAAAAGTAAGCGAATGTGGCTTTCTCTACGAATGGACGGGTCCACAAAAGAAAGAAACAAGACGAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGGCTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCATGGAATAAAATATGCGCTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTGGAGCTTGAACCTCGAATGAGAAAGGCATTTCAAGGACTGAA
TATAGCTGCACTTTATGGCGGGAGTTCTCAAAGGTTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCCTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAATCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAAGATGT
TGCTCGTAGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGTTCAAGATGTAAA
TGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGTATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAGGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
TCAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAACGGAGACGTGTATTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTGAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.676

98.667

0.549
  comFA/cflA Streptococcus pneumoniae Rx1

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae D39

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae R6

40.678

94.4

0.384
  comFA/cflA Streptococcus pneumoniae TIGR4

40.395

94.4

0.381
  comFA Latilactobacillus sakei subsp. sakei 23K

38.587

98.133

0.379
  comFA/cflA Streptococcus mitis NCTC 12261

40.113

94.4

0.379
  comFA/cflA Streptococcus mitis SK321

38.983

94.4

0.368
  comFA Lactococcus lactis subsp. cremoris KW2

37.432

97.6

0.365