Detailed information NTG0710391

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NNL22_RS01435
• Genome accession: NZ_CP101527
• Coordinates: 307071..308192 (+)
• Length: 373 a.a.
• NCBI ID: WP_251810847.1
• Uniprot ID: -
• Organism: Alkalimarinus sediminis strain FA028
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 302071..313192

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NNL22_RS01415 (NNL22_01410)	-	303303..303890 (-)	588	WP_251810853.1	YggT family protein	-
NNL22_RS01420 (NNL22_01415)	proC	304082..304912 (-)	831	WP_251810852.1	pyrroline-5-carboxylate reductase	-
NNL22_RS01425 (NNL22_01420)	-	305065..305760 (-)	696	WP_251810850.1	YggS family pyridoxal phosphate-dependent enzyme	-
NNL22_RS01430 (NNL22_01425)	pilT	305953..306987 (+)	1035	WP_251810848.1	type IV pilus twitching motility protein PilT	Machinery gene
NNL22_RS01435 (NNL22_01430)	pilU	307071..308192 (+)	1122	WP_251810847.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NNL22_RS01440 (NNL22_01435)	polA	308320..311067 (-)	2748	WP_251810846.1	DNA polymerase I	-
NNL22_RS01445 (NNL22_01440)	-	311127..311426 (+)	300	WP_251810844.1	DUF2782 domain-containing protein	-
NNL22_RS01450 (NNL22_01445)	-	311481..312449 (+)	969	WP_251810843.1	homoserine kinase	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41361.49 Da
• Isoelectric Point: 6.1825

>NTDB_id=710391 NNL22_RS01435 WP_251810847.1 307071..308192(+) (pilU) [Alkalimarinus sediminis strain FA028]
MDFDKLLKVMVEKGGSDLFITAGVPPSIKIHGKIVPVTKSSLTPEQTREIVFNTMSEKQREEFTEAHECNFAISARGIGR
FRVSAFYQRNLCGMVLRRIETNIPKVDDLALPEIIKTLSMTKRGLIIFVGATGTGKSTSLASMIGHRNRNSQGHIISIED
PIEYIHQHQGCIVTQREVGIDTESFDVALKNTLRQAPDVIMIGEIRSKDTMDYAVTFAETGHLCLATLHANNANQALDRI
INFFPPEQHNQIWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLCADMIRKAEVHKLKELMTKSRDVGMQTFDQA
LYDLYSAGEITYEDALAYADSANDLRLMIKLGADSSGDKLSASLDGLSINDEI

Nucleotide

• Length: 1122 bp

>NTDB_id=710391 NNL22_RS01435 WP_251810847.1 307071..308192(+) (pilU) [Alkalimarinus sediminis strain FA028]
ATGGACTTCGATAAACTATTAAAAGTAATGGTTGAGAAAGGTGGTTCAGACCTTTTTATTACGGCCGGTGTGCCACCGAG
CATTAAAATTCACGGTAAGATTGTGCCGGTTACTAAGTCTTCATTAACGCCCGAACAGACCCGTGAAATCGTTTTTAATA
CGATGAGTGAAAAACAGCGGGAAGAGTTTACCGAGGCCCATGAGTGTAACTTTGCTATTAGTGCCCGGGGTATTGGTCGT
TTTCGTGTGAGTGCGTTTTATCAGCGTAATCTATGTGGCATGGTATTGCGTCGCATTGAAACCAATATTCCAAAAGTGGA
TGATTTGGCACTGCCAGAGATTATTAAGACCTTGTCGATGACTAAGCGGGGCCTGATTATCTTTGTGGGGGCGACAGGCA
CAGGTAAATCCACTTCGTTAGCATCGATGATTGGTCATCGAAATCGTAATAGCCAAGGGCATATCATCTCGATTGAAGAT
CCGATTGAGTATATTCACCAACACCAAGGTTGCATCGTGACTCAGCGTGAAGTGGGTATAGACACCGAAAGCTTTGATGT
AGCGTTGAAAAATACGCTGCGTCAGGCACCCGACGTGATCATGATCGGTGAGATTCGATCCAAAGATACGATGGACTATG
CAGTAACCTTTGCAGAAACAGGTCACCTCTGTTTAGCAACACTGCACGCTAATAACGCAAACCAGGCGTTGGATAGAATT
ATTAACTTCTTCCCGCCAGAGCAGCATAACCAAATCTGGATGGATTTATCATTAAACCTTAAAGCCATTGTGGCTCAGCA
GTTGGTTCCTACGCCTGATGGCAAGGGGCGAAGAGCTGTAATAGAGGTATTAATCAATACGCCTCTCTGTGCAGATATGA
TTCGTAAGGCAGAGGTCCATAAGCTTAAAGAGCTGATGACTAAATCCCGAGATGTGGGTATGCAGACCTTTGATCAAGCA
TTGTATGATCTGTATTCGGCGGGTGAAATTACCTACGAAGATGCGTTAGCTTACGCTGATTCTGCGAATGATTTGAGGTT
GATGATTAAGCTAGGGGCTGATTCATCAGGTGATAAGCTTTCTGCTTCGTTGGATGGATTATCGATTAATGATGAGATCT
AG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	77.507	98.928	0.767
pilU	Acinetobacter baylyi ADP1	64.986	95.71	0.622
pilU	Vibrio cholerae strain A1552	53.495	99.732	0.534
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.11	97.587	0.391