Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NM966_RS16980 Genome accession   NZ_CP101506
Coordinates   3240030..3241157 (-) Length   375 a.a.
NCBI ID   WP_046526682.1    Uniprot ID   -
Organism   Bacillus altitudinis strain B12     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3235030..3246157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM966_RS16945 (NM966_16890) flgL 3235213..3236124 (-) 912 WP_017368141.1 flagellar hook-associated protein FlgL -
  NM966_RS16950 (NM966_16895) flgK 3236133..3237653 (-) 1521 WP_017368142.1 flagellar hook-associated protein FlgK -
  NM966_RS16955 (NM966_16900) - 3237669..3238151 (-) 483 WP_007499977.1 flagellar protein FlgN -
  NM966_RS16960 (NM966_16905) flgM 3238170..3238433 (-) 264 WP_003213252.1 flagellar biosynthesis anti-sigma factor FlgM -
  NM966_RS16965 (NM966_16910) - 3238513..3238932 (-) 420 WP_017357864.1 TIGR03826 family flagellar region protein -
  NM966_RS16970 (NM966_16915) - 3238989..3239711 (-) 723 WP_231947217.1 ComF family protein -
  NM966_RS16975 (NM966_16920) - 3239672..3239965 (-) 294 WP_035701582.1 late competence development ComFB family protein -
  NM966_RS16980 (NM966_16925) comFA 3240030..3241157 (-) 1128 WP_046526682.1 DEAD/DEAH box helicase Machinery gene
  NM966_RS16985 (NM966_16930) - 3241529..3242371 (-) 843 WP_060832692.1 DegV family protein -
  NM966_RS16990 (NM966_16935) degU 3242583..3243272 (-) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  NM966_RS16995 (NM966_16940) degS 3243337..3244512 (-) 1176 WP_035389673.1 sensor histidine kinase Regulator
  NM966_RS17000 (NM966_16945) - 3244734..3245402 (+) 669 WP_017357860.1 YigZ family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 42838.92 Da        Isoelectric Point: 10.4275

>NTDB_id=710316 NM966_RS16980 WP_046526682.1 3240030..3241157(-) (comFA) [Bacillus altitudinis strain B12]
MLGKVSECGFLYEWTGPQKKETRQVELTWQGELSKGQKRASDRLVEAIQNKFDLLVWAVCGAGKTEVLFHGIKYALNNGM
SVCIATPRTDVVLELEPRMRKAFQGVNIAVLYGGSSQRFQMAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSMDERLQF
AVLKSMKKTGVRIYLSATPSKKMQKDVARGQLEAIKIPLRFHQKPLPVPTFQWVGDWKKKLKRNRLPASTMNWLHEHITK
KKRVLLFVPSISTMKKVTHILQVQCVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTIQDVQVGVLGAESPIF
TESALVQISGRAGRHPDFFNGDVYFFHFGLTRSMKQAKKHIVKMNNTAEKEFSEK

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=710316 NM966_RS16980 WP_046526682.1 3240030..3241157(-) (comFA) [Bacillus altitudinis strain B12]
ATGCTGGGAAAAGTAAGTGAATGTGGCTTTCTCTATGAATGGACGGGTCCACAAAAGAAAGAAACAAGACAAGTTGAATT
AACATGGCAGGGAGAGCTTTCTAAAGGGCAAAAGAGAGCGTCCGATAGACTAGTAGAAGCAATCCAAAACAAATTTGATC
TGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCACGGAATAAAATATGCGTTAAATAACGGAATG
AGTGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTCGAACCTCGAATGAGGAAGGCATTTCAAGGAGTGAA
TATAGCTGTACTTTATGGCGGGAGTTCTCAAAGATTTCAAATGGCACCGCTTATGATCGCTACAACCCATCAGCTAATGA
GATATAAAAACGCTTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCAATGGATGAACGGCTTCAGTTT
GCTGTTTTAAAGTCAATGAAAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAATGCAAAAGGATGT
TGCTCGTGGACAATTAGAAGCGATCAAAATTCCTCTGCGCTTCCACCAAAAACCGTTGCCTGTCCCAACATTTCAATGGG
TTGGAGATTGGAAAAAGAAATTAAAAAGAAATCGGCTTCCAGCATCTACAATGAACTGGCTGCATGAACATATCACAAAG
AAAAAAAGAGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGTTCAATGTGTAAA
TGTACAGGGAGTTTCTGCCGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGATACGATGTATTGG
TTACAACAACTATTCTAGAAAGAGGCGTTACCATTCAAGATGTTCAAGTGGGCGTATTAGGTGCGGAATCTCCTATTTTT
ACAGAAAGTGCACTTGTCCAAATTTCTGGTAGAGCAGGGAGACATCCAGATTTTTTTAATGGAGACGTGTACTTTTTTCA
TTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAACATATCGTAAAAATGAATAATACAGCTGAAAAAGAGTTTTCTG
AAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(32-180)

Helicase conserved C-terminal domain (Helicase_C)

(236-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

55.946

98.667

0.552
  comFA/cflA Streptococcus pneumoniae Rx1

41.525

94.4

0.392
  comFA/cflA Streptococcus pneumoniae D39

41.525

94.4

0.392
  comFA/cflA Streptococcus pneumoniae R6

41.525

94.4

0.392
  comFA/cflA Streptococcus pneumoniae TIGR4

41.243

94.4

0.389
  comFA/cflA Streptococcus mitis NCTC 12261

40.96

94.4

0.387
  comFA Latilactobacillus sakei subsp. sakei 23K

38.172

99.2

0.379
  comFA/cflA Streptococcus mitis SK321

39.831

94.4

0.376
  comFA Lactococcus lactis subsp. cremoris KW2

37.705

97.6

0.368