Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E5Q65_RS19095 Genome accession   NZ_CP116480
Coordinates   3953025..3953465 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain GTEN_24     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3948025..3958465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Q65_RS19070 coaE 3948515..3949135 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  E5Q65_RS19075 - 3949160..3949204 (+) 45 WP_120795372.1 protein YacM -
  E5Q65_RS19080 guaC 3949360..3950403 (+) 1044 WP_001217338.1 GMP reductase -
  E5Q65_RS19085 hofC 3950438..3951640 (-) 1203 WP_000157234.1 protein transport protein HofC -
  E5Q65_RS19090 pilB 3951630..3953015 (-) 1386 WP_001025154.1 type II secretion system protein GspE Machinery gene
  E5Q65_RS19095 pilA 3953025..3953465 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  E5Q65_RS19100 nadC 3953668..3954561 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E5Q65_RS19105 ampD 3954649..3955200 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E5Q65_RS19110 ampE 3955197..3956051 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  E5Q65_RS19115 aroP 3956094..3957467 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=710317 E5Q65_RS19095 WP_000360904.1 3953025..3953465(-) (pilA) [Escherichia coli strain GTEN_24]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=710317 E5Q65_RS19095 WP_000360904.1 3953025..3953465(-) (pilA) [Escherichia coli strain GTEN_24]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363