Detailed information NTG0709633

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PIG43_RS14505
• Genome accession: NZ_CP116387
• Coordinates: 2994819..2995937 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain WM98
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2989819..3000937

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PIG43_RS14495 (PIG43_14465)	-	2992935..2993627 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
PIG43_RS14500 (PIG43_14470)	pilT	2993754..2994791 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
PIG43_RS14505 (PIG43_14475)	pilU	2994819..2995937 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PIG43_RS14510 (PIG43_14480)	fur	2996007..2996444 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
PIG43_RS14515 (PIG43_14485)	bamE	2996556..2996954 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
PIG43_RS14520 (PIG43_14490)	-	2997009..2997332 (-)	324	WP_000442585.1	RnfH family protein	-
PIG43_RS14525 (PIG43_14495)	-	2997332..2998399 (-)	1068	WP_000092403.1	hypothetical protein	-
PIG43_RS14530 (PIG43_14500)	-	2998552..2999007 (-)	456	WP_000782976.1	bacteriohemerythrin	-
PIG43_RS14535 (PIG43_14505)	-	2999260..3000108 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=709633 PIG43_RS14505 WP_000347036.1 2994819..2995937(+) (pilU) [Acinetobacter baumannii strain WM98]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=709633 PIG43_RS14505 WP_000347036.1 2994819..2995937(+) (pilU) [Acinetobacter baumannii strain WM98]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392