Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NKT77_RS08475 Genome accession   NZ_CP101111
Coordinates   1871005..1872657 (-) Length   550 a.a.
NCBI ID   WP_254729267.1    Uniprot ID   -
Organism   Moraxella sp. FZLJ2107     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1866005..1877657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKT77_RS08455 (NKT77_08455) - 1866720..1868012 (-) 1293 WP_254729260.1 alpha-hydroxy-acid oxidizing protein -
  NKT77_RS08460 (NKT77_08460) coaE 1868255..1868881 (-) 627 WP_254729262.1 dephospho-CoA kinase -
  NKT77_RS08465 (NKT77_08465) - 1868923..1869780 (-) 858 WP_254732157.1 A24 family peptidase -
  NKT77_RS08470 (NKT77_08470) - 1869780..1871000 (-) 1221 WP_254729264.1 type II secretion system F family protein -
  NKT77_RS08475 (NKT77_08475) pilB 1871005..1872657 (-) 1653 WP_254729267.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NKT77_RS08480 (NKT77_08480) tpiA 1872835..1873590 (+) 756 WP_254732159.1 triose-phosphate isomerase -
  NKT77_RS08485 (NKT77_08485) secG 1873639..1873959 (+) 321 WP_254729269.1 preprotein translocase subunit SecG -
  NKT77_RS08495 (NKT77_08495) - 1874309..1875202 (-) 894 WP_254729272.1 Fic family protein -
  NKT77_RS08505 (NKT77_08505) rimP 1875870..1876367 (+) 498 WP_253224441.1 ribosome maturation factor RimP -

Sequence

Protein


Download         Length: 550 a.a.        Molecular weight: 60329.02 Da        Isoelectric Point: 6.3241

>NTDB_id=708691 NKT77_RS08475 WP_254729267.1 1871005..1872657(-) (pilB) [Moraxella sp. FZLJ2107]
MLTAFHHRLIAQLSQLGCDADALTTIMTDAYANSTPWTDAVINAQLVSPKLLGDTMAILLNMPLVDLDRHDTRDAGTLID
PKLIARHRVLPLSKDNSTLTLAMSDPTDRDAIQSIRFHTHLNVTPVLVEADKLSRHLDKLSLGAPVVLDEHLDDEDIEDD
PIVDAPTVQFVQKILADAVQLGASDIHFEPYENSFRVRYRIDGVMSSVNTPPKSQAGKITTRLKVMAHLDIAERRKPQDG
RIKFRLSEHKSVDFRVSTLPTLFGEKVVLRILDSAQTLIGMDALGLNATQKQQFTDALHKPQGMILITGPTGSGKTISLY
TALGLLNRGDNNILTAEDPVEINLDGINQTNINPKIGLDFADLLRAFLRQDPDVIMVGEIRDTETAEIAIKAAQTGHLVL
STLHTNSAAATLTRLGSMGVPSFQLASGVNLIIAQRLARRLCDDCKRAVVLPPQVLREAGFGDDDISRTHQLYEPVGCHR
CRDGYRGRVGIFELLPITPNIRKLIFDRADSDVIDHANRQAGNISLRQSGIAAVMAGITSLTEMHRATSD

Nucleotide


Download         Length: 1653 bp        

>NTDB_id=708691 NKT77_RS08475 WP_254729267.1 1871005..1872657(-) (pilB) [Moraxella sp. FZLJ2107]
ATGCTGACCGCGTTTCATCATCGACTCATTGCACAGTTATCACAGCTTGGTTGTGATGCTGACGCGCTCACCACCATCAT
GACTGATGCATATGCCAACTCTACCCCATGGACAGATGCTGTAATCAATGCACAGCTTGTCAGCCCAAAGCTGCTTGGTG
ATACGATGGCGATACTGCTCAATATGCCACTTGTCGATTTGGATCGTCATGATACGCGCGATGCTGGCACGCTGATCGAT
CCTAAGCTCATTGCGCGCCATCGCGTCTTGCCACTTAGCAAGGATAATAGCACGCTGACCCTTGCCATGAGCGATCCGAC
TGACCGCGATGCCATTCAGTCTATTCGCTTTCACACACATCTGAATGTCACGCCAGTACTGGTTGAAGCTGACAAATTGT
CGCGACATCTTGATAAGCTGAGTCTGGGCGCGCCCGTGGTGCTCGATGAACATCTGGATGATGAAGACATCGAAGACGAT
CCCATCGTTGATGCGCCGACGGTACAATTTGTCCAAAAAATCCTAGCCGACGCGGTGCAACTGGGCGCATCTGACATTCA
TTTTGAGCCATATGAAAACAGCTTTCGCGTGCGCTATCGCATCGATGGCGTGATGAGCAGTGTCAATACACCACCCAAAA
GTCAAGCGGGTAAAATCACCACGCGGCTTAAAGTCATGGCGCATTTGGACATCGCCGAGCGACGCAAACCCCAAGATGGG
CGTATCAAATTTCGCTTATCTGAACATAAATCGGTGGATTTTCGCGTCAGTACTTTGCCTACTTTATTCGGTGAAAAAGT
CGTCTTACGAATCTTGGACTCAGCGCAGACGCTCATCGGCATGGACGCGCTAGGGCTTAATGCCACACAAAAACAGCAGT
TCACCGACGCCCTGCACAAGCCCCAAGGCATGATTTTGATCACAGGTCCGACAGGCAGCGGCAAGACCATCTCGCTCTAT
ACCGCGCTTGGGTTATTAAACCGTGGCGATAATAATATCTTGACCGCTGAAGATCCTGTGGAAATCAATCTTGACGGCAT
TAACCAGACTAACATCAATCCAAAAATCGGCTTGGATTTTGCCGACTTATTACGCGCGTTCTTACGCCAAGATCCTGATG
TGATCATGGTCGGTGAAATTCGCGATACCGAGACGGCTGAGATCGCGATCAAGGCAGCACAAACAGGGCATTTGGTGCTA
TCGACCCTGCATACCAATTCTGCCGCTGCCACCTTGACGCGTCTTGGCAGTATGGGCGTGCCAAGCTTTCAACTTGCCAG
TGGCGTCAATCTCATCATCGCCCAACGCCTAGCGCGGCGGCTGTGTGATGACTGCAAGCGCGCGGTAGTACTACCGCCAC
AGGTGCTGCGTGAAGCAGGCTTTGGTGATGATGACATCTCACGCACCCATCAGCTGTACGAGCCTGTGGGCTGCCATCGC
TGCCGTGATGGCTATCGTGGGCGCGTGGGTATCTTTGAGCTGCTGCCGATCACGCCCAATATTCGAAAGTTGATTTTTGA
TCGTGCTGACAGCGATGTCATTGATCATGCCAATCGCCAAGCAGGTAATATCAGCTTGCGCCAATCAGGAATCGCCGCTG
TCATGGCAGGCATCACAAGCTTGACTGAAATGCACCGCGCGACATCGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-140)

Type II/IV secretion system protein (T2SSE)

(173-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.064

100

0.524
  pilB Acinetobacter baylyi ADP1

52.562

95.818

0.504
  pilB Legionella pneumophila strain ERS1305867

50.275

99.091

0.498
  pilB Vibrio cholerae strain A1552

46.95

98.364

0.462
  pilF Neisseria gonorrhoeae MS11

47.048

95.455

0.449
  pilB Vibrio campbellii strain DS40M4

45.572

98.545

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

44.815

98.182

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.476

95.455

0.367
  pilF Thermus thermophilus HB27

38.269

94.545

0.362