Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   PIC71_RS00340 Genome accession   NZ_CP116152
Coordinates   69538..70206 (+) Length   222 a.a.
NCBI ID   WP_001350561.1    Uniprot ID   B1X972
Organism   Escherichia coli strain DETEC-P61     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 64538..75206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIC71_RS00310 (PIC71_00310) yicC 64557..65420 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  PIC71_RS00315 (PIC71_00315) rph 65547..66263 (+) 717 WP_001247097.1 ribonuclease PH -
  PIC71_RS00320 (PIC71_00320) pyrE 66329..66970 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  PIC71_RS00325 (PIC71_00325) slmA 67007..67603 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  PIC71_RS00330 (PIC71_00330) dut 67710..68168 (-) 459 WP_000976073.1 dUTP diphosphatase -
  PIC71_RS00335 (PIC71_00335) coaBC 68146..69366 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  PIC71_RS00340 (PIC71_00340) radC 69538..70206 (+) 669 WP_001350561.1 RadC family protein Machinery gene
  PIC71_RS00345 (PIC71_00345) rpmB 70423..70659 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  PIC71_RS00350 (PIC71_00350) rpmG 70680..70847 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  PIC71_RS00355 (PIC71_00355) mutM 70945..71754 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  PIC71_RS00360 (PIC71_00360) coaD 71793..72272 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  PIC71_RS00365 (PIC71_00365) waaA 72280..73557 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  PIC71_RS00370 (PIC71_00370) rfaQ 73970..75028 (+) 1059 WP_000360406.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25343.46 Da        Isoelectric Point: 8.4958

>NTDB_id=708386 PIC71_RS00340 WP_001350561.1 69538..70206(+) (radC) [Escherichia coli strain DETEC-P61]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHRRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=708386 PIC71_RS00340 WP_001350561.1 69538..70206(+) (radC) [Escherichia coli strain DETEC-P61]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGGCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1X972

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496