Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   NMD25_RS21875 Genome accession   NZ_CP101010
Coordinates   4516729..4518249 (+) Length   506 a.a.
NCBI ID   WP_219358889.1    Uniprot ID   -
Organism   Escherichia coli strain ET76     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4511729..4523249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD25_RS21850 (NMD25_21715) ilvE 4513283..4514212 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  NMD25_RS21855 (NMD25_21720) ilvM 4514232..4514495 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  NMD25_RS21860 (NMD25_21725) ilvG 4514492..4516138 (-) 1647 WP_001012608.1 acetolactate synthase 2 catalytic subunit -
  NMD25_RS21865 (NMD25_21730) ilvX 4516141..4516191 (-) 51 WP_001387183.1 peptide IlvX -
  NMD25_RS21870 (NMD25_21735) ilvL 4516278..4516376 (-) 99 WP_001311244.1 ilv operon leader peptide -
  NMD25_RS21875 (NMD25_21740) comM 4516729..4518249 (+) 1521 WP_219358889.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  NMD25_RS21880 (NMD25_21745) maoP 4518274..4518612 (-) 339 WP_074507223.1 macrodomain Ori organization protein MaoP -
  NMD25_RS21885 (NMD25_21750) hdfR 4518731..4519570 (+) 840 WP_032235330.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55170.37 Da        Isoelectric Point: 7.3875

>NTDB_id=708122 NMD25_RS21875 WP_219358889.1 4516729..4518249(+) (comM) [Escherichia coli strain ET76]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPLRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=708122 NMD25_RS21875 WP_219358889.1 4516729..4518249(+) (comM) [Escherichia coli strain ET76]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTGAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTTGCTCCAGCCGATCTACCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCTCTTACAGGCGCTCTGCGTGGCGTTCCCGGTGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAACAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTACG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGTTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGTTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAGCAAACGCTGCGTTATCTCAACCGACTCTCTGGTC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCTCCCGGTATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCGCCACCGTTAAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAACGCCTG
GCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAACATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.513

100

0.591
  comM Vibrio cholerae strain A1552

59.163

99.209

0.587
  comM Glaesserella parasuis strain SC1401

58.383

100

0.585
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Legionella pneumophila str. Paris

47.992

98.419

0.472
  comM Legionella pneumophila strain ERS1305867

47.992

98.419

0.472
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.071

100

0.441