Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   PIC52_RS18450 Genome accession   NZ_CP116100
Coordinates   3811419..3812912 (+) Length   497 a.a.
NCBI ID   WP_000249335.1    Uniprot ID   -
Organism   Escherichia coli strain DETEC-P881     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3806419..3817912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIC52_RS18430 (PIC52_18430) pilD 3807067..3807876 (+) 810 WP_001606131.1 prepilin peptidase PppA Machinery gene
  PIC52_RS18435 (PIC52_18435) gspS2 3807942..3808352 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  PIC52_RS18440 (PIC52_18440) gspC 3808370..3809329 (+) 960 WP_001606130.1 type II secretion system protein GspC -
  PIC52_RS18445 (PIC52_18445) gspD 3809359..3811419 (+) 2061 WP_000498844.1 type II secretion system secretin GspD -
  PIC52_RS18450 (PIC52_18450) pilB/pilB1 3811419..3812912 (+) 1494 WP_000249335.1 type II secretion system ATPase GspE Machinery gene
  PIC52_RS18455 (PIC52_18455) gspF 3812912..3814135 (+) 1224 WP_001173412.1 type II secretion system inner membrane protein GspF -
  PIC52_RS18460 (PIC52_18460) gspG 3814152..3814607 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  PIC52_RS18465 (PIC52_18465) gspH 3814611..3815174 (+) 564 WP_001606128.1 type II secretion system minor pseudopilin GspH -
  PIC52_RS18470 (PIC52_18470) gspI 3815171..3815542 (+) 372 WP_000820087.1 type II secretion system minor pseudopilin GspI -
  PIC52_RS18475 (PIC52_18475) gspJ 3815539..3816144 (+) 606 WP_001250459.1 type II secretion system minor pseudopilin GspJ -
  PIC52_RS18480 (PIC52_18480) gspK 3816144..3817121 (+) 978 WP_024193741.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55129.20 Da        Isoelectric Point: 5.9800

>NTDB_id=707788 PIC52_RS18450 WP_000249335.1 3811419..3812912(+) (pilB/pilB1) [Escherichia coli strain DETEC-P881]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCSDCRQAAPA
TDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=707788 PIC52_RS18450 WP_000249335.1 3811419..3812912(+) (pilB/pilB1) [Escherichia coli strain DETEC-P881]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCAACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTGAGTCACGAACTGACC
GCGCAGTTGCGTCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACCCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTTTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCTCCGACTGCCGTCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Acinetobacter baumannii D1279779

47.118

80.282

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilB Acinetobacter baylyi ADP1

46.667

78.471

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362