Detailed information NTG0070497

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EP181_RS08970
• Genome accession: NZ_AP018722
• Coordinates: 1956231..1957388 (+)
• Length: 385 a.a.
• NCBI ID: WP_127471336.1
• Uniprot ID: -
• Organism: Thiomicrorhabdus aquaedulcis strain HaS4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1951231..1962388

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EP181_RS08950	lptF	1951851..1952887 (-)	1037	Protein_1814	LPS export ABC transporter permease LptF	-
EP181_RS08955	-	1953114..1954613 (+)	1500	WP_127471333.1	leucyl aminopeptidase	-
EP181_RS08960	-	1954623..1955126 (+)	504	WP_127471334.1	DNA polymerase III subunit chi	-
EP181_RS08965	pilT	1955173..1956210 (+)	1038	WP_127471335.1	type IV pilus twitching motility protein PilT	Machinery gene
EP181_RS08970	pilU	1956231..1957388 (+)	1158	WP_127471336.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EP181_RS08975	-	1957480..1957920 (-)	441	WP_127471337.1	type II toxin-antitoxin system VapC family toxin	-
EP181_RS08980	-	1957914..1958228 (-)	315	WP_127471338.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EP181_RS08985	uvrB	1958355..1960397 (-)	2043	WP_127471339.1	excinuclease ABC subunit UvrB	-
EP181_RS08990	-	1960550..1961731 (+)	1182	WP_127471340.1	pyridoxal phosphate-dependent aminotransferase	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42656.63 Da
• Isoelectric Point: 6.3865

>NTDB_id=70497 EP181_RS08970 WP_127471336.1 1956231..1957388(+) (pilU) [Thiomicrorhabdus aquaedulcis strain HaS4]
MNNDTLNDLHALLTYFSQQGGSDLFITAGRPASMKKDGKIIDITEEKLTPAIATQLTHAIMNHDQLASFQTTQECNFAYH
ISGLARYRVNAFIQRGTPGLVIRSVHTHIPSFESLKLPPILRDIILEKRGLVLIVGGTGSGKSTTLASLIDVRNRETQGH
IITIEDPIEFIHSHQNCIVTQREVGVDTDSYSTALKNTLRQAPDVILIGEIRSRETMEHAIAFSETGHLCLSTLHANNAN
QTLDRIVNFFSSAERAHILMDLSLNIKAIVSQRLVPKIGGGLVAAIEVLINTPRMAELIFNGKVGEIKQLMLASEAQGMQ
TFDQALFELYEAQKITYEDALRNADSTNDLRLNIKLNSQLPPPNAEYEEQKAQPTPTFSLRTHVD

Nucleotide

• Length: 1158 bp

>NTDB_id=70497 EP181_RS08970 WP_127471336.1 1956231..1957388(+) (pilU) [Thiomicrorhabdus aquaedulcis strain HaS4]
ATGAACAATGACACCTTAAACGATTTGCACGCCTTACTGACCTACTTTAGCCAGCAAGGTGGCTCCGATTTATTCATTAC
CGCCGGTCGCCCCGCGTCCATGAAAAAAGACGGCAAAATTATCGACATTACCGAAGAAAAACTCACCCCTGCCATTGCCA
CTCAACTGACTCACGCCATTATGAACCACGACCAACTGGCGTCGTTTCAGACCACCCAAGAGTGCAACTTTGCTTATCAC
ATCAGTGGGCTGGCGCGGTATCGGGTTAACGCGTTTATTCAACGCGGCACACCAGGCCTGGTCATTCGTTCGGTACACAC
GCACATTCCGTCGTTTGAAAGCTTAAAGTTGCCGCCCATTTTGCGCGACATTATTTTAGAAAAACGCGGTCTAGTATTAA
TTGTCGGTGGCACAGGCTCAGGTAAATCGACCACACTGGCCAGCTTAATTGACGTACGCAATCGAGAAACCCAAGGCCAC
ATTATTACCATTGAAGACCCCATTGAGTTTATTCACTCACATCAAAACTGCATTGTGACCCAACGCGAAGTGGGCGTGGA
CACCGACAGCTACTCAACCGCCCTTAAAAACACCTTGCGCCAAGCACCCGACGTGATTTTAATTGGTGAAATTCGCAGCC
GCGAAACCATGGAACATGCCATTGCGTTTTCAGAAACCGGTCATTTATGCCTGTCCACCCTGCACGCCAACAACGCAAAC
CAAACGCTTGACCGCATTGTTAACTTTTTTTCATCCGCCGAGCGCGCCCATATTTTGATGGACTTATCGCTCAACATCAA
AGCCATTGTGTCGCAACGATTAGTGCCTAAAATCGGCGGCGGCTTGGTGGCCGCCATTGAAGTATTAATCAACACCCCGC
GCATGGCCGAGTTAATTTTTAATGGCAAGGTGGGAGAGATAAAACAGTTGATGTTGGCCTCAGAAGCCCAAGGCATGCAA
ACGTTTGACCAAGCCTTATTTGAACTGTATGAAGCGCAAAAAATCACTTACGAAGACGCTCTGCGCAACGCCGACTCCAC
CAACGATTTACGCTTAAACATCAAACTAAACAGCCAGCTGCCGCCCCCCAACGCCGAATACGAAGAACAAAAAGCCCAAC
CCACTCCCACGTTTAGCCTACGCACTCACGTTGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (119-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.34	91.688	0.553
pilU	Acinetobacter baylyi ADP1	55.307	92.987	0.514
pilU	Vibrio cholerae strain A1552	54.762	87.273	0.478
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.42	87.792	0.364