NTDB

Overview

Name	comE	Type	Machinery gene
Locus tag	PCS90_RS22015	Genome accession	NZ_CP115386
Coordinates	4477008..4478246 (-)	Length	412 a.a.
NCBI ID	WP_000816006.1	Uniprot ID	-
Organism	Escherichia coli strain CUVET20-PYO2
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4472008..4483246

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCS90_RS21995 (PCS90_21940)	dam	4472620..4473456 (-)	837	WP_000742141.1	adenine-specific DNA-methyltransferase	-
PCS90_RS22000 (PCS90_21945)	damX	4473563..4474849 (-)	1287	WP_000343176.1	cell division protein DamX	-
PCS90_RS22005 (PCS90_21950)	aroB	4474941..4476029 (-)	1089	WP_001356903.1	3-dehydroquinate synthase	-
PCS90_RS22010 (PCS90_21955)	aroK	4476086..4476607 (-)	522	WP_000818618.1	shikimate kinase AroK	-
PCS90_RS22015 (PCS90_21960)	comE	4477008..4478246 (-)	1239	WP_000816006.1	DNA uptake porin HofQ	Machinery gene
PCS90_RS22020 (PCS90_21965)	hofP	4478158..4478562 (-)	405	WP_001264138.1	DNA utilization protein HofP	-
PCS90_RS22025 (PCS90_21970)	hofO	4478552..4478992 (-)	441	WP_001055744.1	DNA utilization protein HofO	-
PCS90_RS22030 (PCS90_21975)	hofN	4478976..4479515 (-)	540	WP_001069307.1	DNA utilization protein HofN	-
PCS90_RS22035 (PCS90_21980)	hofM	4479515..4480294 (-)	780	WP_001296474.1	DNA utilization protein HofM	-
PCS90_RS22040 (PCS90_21985)	mrcA	4480414..4482966 (+)	2553	WP_001298208.1	peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA	-

Sequence

Protein

Download Length: 412 a.a. Molecular weight: 44797.26 Da Isoelectric Point: 5.9488

                                >NTDB_id=704129 PCS90_RS22015 WP_000816006.1 4477008..4478246(-) (comE) [Escherichia coli strain CUVET20-PYO2]

MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL

ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN

KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN

GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL

HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL

VVFITPRLVSSE

Nucleotide

Download Length: 1239 bp

                                >NTDB_id=704129 PCS90_RS22015 WP_000816006.1 4477008..4478246(-) (comE) [Escherichia coli strain CUVET20-PYO2]

ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT

GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG

TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG

ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA

GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA

AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC

AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT

TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG

GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC

GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC

CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT

CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA

CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTACTGGCGATTGATAAGCAGGAGATCGA

AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA

GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA

GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comE	Haemophilus influenzae 86-028NP	37.3	100	0.396
pilQ	Vibrio campbellii strain DS40M4	38.48	100	0.393
comE	Haemophilus influenzae Rd KW20	37.156	100	0.393
pilQ	Vibrio cholerae strain A1552	37.772	100	0.379
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	37.772	100	0.379
comE	Glaesserella parasuis strain SC1401	35.782	100	0.367
pilQ	Pseudomonas aeruginosa PAK	34.633	100	0.367

Detailed information NTG0704129