Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   PCS86_RS22095 Genome accession   NZ_CP115343
Coordinates   4491501..4492739 (-) Length   412 a.a.
NCBI ID   WP_000816006.1    Uniprot ID   -
Organism   Escherichia coli strain CUVET21-H2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4486501..4497739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCS86_RS22075 (PCS86_22010) dam 4487113..4487949 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  PCS86_RS22080 (PCS86_22015) damX 4488056..4489342 (-) 1287 WP_000343176.1 cell division protein DamX -
  PCS86_RS22085 (PCS86_22020) aroB 4489434..4490522 (-) 1089 WP_001356903.1 3-dehydroquinate synthase -
  PCS86_RS22090 (PCS86_22025) aroK 4490579..4491100 (-) 522 WP_000818618.1 shikimate kinase AroK -
  PCS86_RS22095 (PCS86_22030) comE 4491501..4492739 (-) 1239 WP_000816006.1 DNA uptake porin HofQ Machinery gene
  PCS86_RS22100 (PCS86_22035) hofP 4492651..4493055 (-) 405 WP_001264138.1 DNA utilization protein HofP -
  PCS86_RS22105 (PCS86_22040) hofO 4493045..4493485 (-) 441 WP_001055744.1 DNA utilization protein HofO -
  PCS86_RS22110 (PCS86_22045) hofN 4493469..4494008 (-) 540 WP_001069307.1 DNA utilization protein HofN -
  PCS86_RS22115 (PCS86_22050) hofM 4494008..4494787 (-) 780 WP_001296474.1 DNA utilization protein HofM -
  PCS86_RS22120 (PCS86_22055) mrcA 4494907..4497459 (+) 2553 WP_001298208.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44797.26 Da        Isoelectric Point: 5.9488

>NTDB_id=703560 PCS86_RS22095 WP_000816006.1 4491501..4492739(-) (comE) [Escherichia coli strain CUVET21-H2]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=703560 PCS86_RS22095 WP_000816006.1 4491501..4492739(-) (comE) [Escherichia coli strain CUVET21-H2]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTACTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.782

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367