Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VRUMOI_RS01175 Genome accession   NZ_AP018685
Coordinates   264619..265203 (+) Length   194 a.a.
NCBI ID   WP_089140077.1    Uniprot ID   -
Organism   Vibrio rumoiensis strain FERM P-14531     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259619..270203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VRUMOI_RS01160 - 260236..262800 (-) 2565 WP_089139975.1 penicillin-binding protein 1A -
  VRUMOI_RS01165 pilM 263023..264039 (+) 1017 WP_089139976.1 type IV pilus assembly protein PilM Machinery gene
  VRUMOI_RS01170 pilN 264029..264619 (+) 591 WP_089139977.1 PilN domain-containing protein Machinery gene
  VRUMOI_RS01175 pilO 264619..265203 (+) 585 WP_089140077.1 type 4a pilus biogenesis protein PilO Machinery gene
  VRUMOI_RS01180 pilP 265200..265709 (+) 510 WP_089139978.1 pilus assembly protein PilP Machinery gene
  VRUMOI_RS01185 pilQ 265752..267548 (+) 1797 WP_231897470.1 type IV pilus secretin PilQ family protein Machinery gene
  VRUMOI_RS01190 aroK 267791..268309 (+) 519 WP_089139979.1 shikimate kinase AroK -
  VRUMOI_RS01195 aroB 268334..269416 (+) 1083 WP_089139980.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22545.85 Da        Isoelectric Point: 4.7645

>NTDB_id=70351 VRUMOI_RS01175 WP_089140077.1 264619..265203(+) (pilO) [Vibrio rumoiensis strain FERM P-14531]
MIDFRDLDFEDMPEWPLLPQLLVIGALIIVLQVVGAWFYLSPQNDELEQIRQQEVTLKSSIRIKAAKVAALPQLQAQLDE
LNTRYQYLLKQLPVQKELASMLSSVNDIGIKNDLSFTRVDWGEKQVQEFLYRLPLNIELTGDYNDIGHFSEAIAKLPRII
SLHNVEWQRVSQESSTLHFRVRAYTYQFKPEEGQ

Nucleotide


Download         Length: 585 bp        

>NTDB_id=70351 VRUMOI_RS01175 WP_089140077.1 264619..265203(+) (pilO) [Vibrio rumoiensis strain FERM P-14531]
ATGATTGATTTTAGAGACCTTGATTTTGAAGATATGCCAGAGTGGCCGCTGTTACCTCAATTGTTAGTGATTGGGGCTTT
AATTATCGTTCTACAAGTTGTTGGAGCATGGTTTTATTTATCTCCACAAAATGATGAGCTTGAACAAATACGCCAGCAAG
AAGTAACACTTAAAAGTTCAATTCGAATTAAAGCTGCAAAAGTGGCAGCGTTACCACAACTGCAAGCGCAGCTGGATGAA
TTAAATACTCGTTATCAATACTTATTAAAGCAGCTACCGGTTCAAAAAGAGTTGGCGAGTATGCTGTCATCCGTTAATGA
CATCGGGATCAAAAACGATCTGAGTTTTACTCGTGTTGATTGGGGGGAGAAACAAGTACAGGAATTTCTCTATCGCCTCC
CTCTTAATATTGAATTAACGGGAGATTATAACGATATCGGTCATTTCTCTGAAGCGATAGCTAAATTACCACGCATTATT
AGTTTACATAACGTGGAGTGGCAACGAGTGAGTCAGGAGAGCAGTACTTTGCACTTTCGTGTTCGTGCTTATACCTACCA
GTTCAAACCGGAGGAAGGGCAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

65.445

98.454

0.644
  pilO Vibrio campbellii strain DS40M4

63.158

97.938

0.619

Multiple sequence alignment