Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VRUMOI_RS01170 Genome accession   NZ_AP018685
Coordinates   264029..264619 (+) Length   196 a.a.
NCBI ID   WP_089139977.1    Uniprot ID   -
Organism   Vibrio rumoiensis strain FERM P-14531     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 259029..269619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VRUMOI_RS01155 oxyR 259244..260137 (+) 894 WP_089139974.1 DNA-binding transcriptional regulator OxyR -
  VRUMOI_RS01160 - 260236..262800 (-) 2565 WP_089139975.1 penicillin-binding protein 1A -
  VRUMOI_RS01165 pilM 263023..264039 (+) 1017 WP_089139976.1 type IV pilus assembly protein PilM Machinery gene
  VRUMOI_RS01170 pilN 264029..264619 (+) 591 WP_089139977.1 PilN domain-containing protein Machinery gene
  VRUMOI_RS01175 pilO 264619..265203 (+) 585 WP_089140077.1 type 4a pilus biogenesis protein PilO Machinery gene
  VRUMOI_RS01180 pilP 265200..265709 (+) 510 WP_089139978.1 pilus assembly protein PilP Machinery gene
  VRUMOI_RS01185 pilQ 265752..267548 (+) 1797 WP_231897470.1 type IV pilus secretin PilQ family protein Machinery gene
  VRUMOI_RS01190 aroK 267791..268309 (+) 519 WP_089139979.1 shikimate kinase AroK -
  VRUMOI_RS01195 aroB 268334..269416 (+) 1083 WP_089139980.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22787.15 Da        Isoelectric Point: 6.6535

>NTDB_id=70350 VRUMOI_RS01170 WP_089139977.1 264029..264619(+) (pilN) [Vibrio rumoiensis strain FERM P-14531]
MLHDINLLPWREEKRQEYKQRFYGMVVLGILAALALQWLASLYIDHLKDQQRHRNQQLQAYITKLDQQLVNLKEIEAKHK
SILTRLRLVESLQNDRNKTTDFMNLMPELVPEGVYVDKIKMSGREIEMAGVSDSTARLATMLDNFESSTWLSDVEMHSII
SGKVLFGKKFQSFKLSFRFEEDEPNESPTIAQQGGQ

Nucleotide


Download         Length: 591 bp        

>NTDB_id=70350 VRUMOI_RS01170 WP_089139977.1 264029..264619(+) (pilN) [Vibrio rumoiensis strain FERM P-14531]
ATGTTGCATGACATTAACCTTTTGCCTTGGAGAGAAGAAAAGCGGCAAGAATATAAGCAACGCTTTTACGGCATGGTGGT
TTTGGGGATTTTAGCGGCTTTAGCGCTGCAGTGGTTGGCCAGTCTCTATATTGATCATTTAAAAGATCAGCAAAGACATC
GAAATCAGCAATTGCAGGCTTATATCACTAAGTTAGATCAACAATTGGTGAACCTGAAAGAAATTGAAGCTAAGCATAAA
TCTATATTGACGCGATTGAGACTGGTTGAGTCACTGCAAAATGATCGAAATAAAACCACCGACTTCATGAACTTAATGCC
GGAGTTAGTTCCTGAAGGTGTTTATGTCGACAAAATAAAAATGAGTGGTCGAGAGATTGAGATGGCAGGAGTCAGTGATA
GCACCGCTAGGTTGGCCACTATGCTTGATAACTTTGAAAGCTCGACGTGGTTGTCTGATGTCGAAATGCATTCGATTATT
TCAGGGAAAGTATTGTTTGGTAAGAAATTTCAAAGCTTCAAGTTGTCGTTTCGCTTTGAAGAAGATGAACCAAATGAGAG
CCCGACGATAGCTCAGCAAGGGGGTCAATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

55.155

98.98

0.546
  pilN Vibrio campbellii strain DS40M4

56.354

92.347

0.52

Multiple sequence alignment