Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VRUMOI_RS01165 Genome accession   NZ_AP018685
Coordinates   263023..264039 (+) Length   338 a.a.
NCBI ID   WP_089139976.1    Uniprot ID   -
Organism   Vibrio rumoiensis strain FERM P-14531     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 258023..269039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VRUMOI_RS01155 oxyR 259244..260137 (+) 894 WP_089139974.1 DNA-binding transcriptional regulator OxyR -
  VRUMOI_RS01160 - 260236..262800 (-) 2565 WP_089139975.1 penicillin-binding protein 1A -
  VRUMOI_RS01165 pilM 263023..264039 (+) 1017 WP_089139976.1 type IV pilus assembly protein PilM Machinery gene
  VRUMOI_RS01170 pilN 264029..264619 (+) 591 WP_089139977.1 PilN domain-containing protein Machinery gene
  VRUMOI_RS01175 pilO 264619..265203 (+) 585 WP_089140077.1 type 4a pilus biogenesis protein PilO Machinery gene
  VRUMOI_RS01180 pilP 265200..265709 (+) 510 WP_089139978.1 pilus assembly protein PilP Machinery gene
  VRUMOI_RS01185 pilQ 265752..267548 (+) 1797 WP_231897470.1 type IV pilus secretin PilQ family protein Machinery gene
  VRUMOI_RS01190 aroK 267791..268309 (+) 519 WP_089139979.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37691.29 Da        Isoelectric Point: 5.8931

>NTDB_id=70349 VRUMOI_RS01165 WP_089139976.1 263023..264039(+) (pilM) [Vibrio rumoiensis strain FERM P-14531]
MGKSFVTGIDIGHHSIKAVVMKPAGELFELVSYKEVPATEGIFSDNHTFNHQEIVKKLKELKKQLPMMAKNVVLSVPDNA
VISKVLHIDSELEEREKEFAIYQVFGQQSPFPVEELSLDYVKVESETLKRAPTSSYQVYATRKEVIESRMEAVKKSGFKP
IVMDIQAHGLLRVWQLATQLDSSKESWLLIDIGQSQTSVCIEPNNRPAYYKDLPFGTQNIKPSKALSMDEASPSLDLAQQ
DEFIIELSERLTRQLSMYSSVNQQQKIEGLWLTGGGATLAAIDEHLSEKLNLPVEILDPLALLQSKVKNKIDAEPCTFSV
AAGLAIRGIQWQGGKHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=70349 VRUMOI_RS01165 WP_089139976.1 263023..264039(+) (pilM) [Vibrio rumoiensis strain FERM P-14531]
ATGGGTAAATCTTTCGTCACGGGCATTGATATTGGCCACCACAGTATAAAAGCTGTGGTCATGAAACCCGCTGGAGAACT
GTTTGAACTGGTGAGTTATAAGGAAGTGCCAGCAACAGAAGGTATTTTTTCAGATAACCACACCTTTAATCATCAAGAAA
TTGTCAAAAAATTAAAAGAGTTAAAAAAGCAGCTGCCGATGATGGCAAAAAATGTGGTGCTTTCGGTACCAGATAACGCT
GTTATTAGCAAAGTATTGCACATTGATAGTGAGCTTGAAGAGCGAGAGAAAGAGTTTGCCATTTATCAAGTCTTCGGCCA
ACAGTCTCCTTTTCCTGTTGAAGAGCTCAGCTTAGATTATGTCAAAGTTGAATCTGAGACTTTAAAACGAGCCCCTACGT
CAAGTTATCAAGTTTATGCTACTCGCAAGGAAGTCATAGAAAGTCGCATGGAAGCCGTAAAAAAGTCGGGTTTCAAACCT
ATTGTGATGGATATTCAGGCGCATGGTCTGTTGCGAGTATGGCAATTAGCGACTCAATTAGATTCAAGTAAAGAAAGCTG
GTTATTAATCGATATTGGTCAAAGCCAAACCTCGGTGTGTATCGAGCCGAATAATCGTCCTGCTTATTACAAAGATCTTC
CTTTTGGTACACAAAATATTAAGCCATCAAAAGCCTTGAGTATGGATGAGGCTTCACCAAGTTTAGATCTCGCTCAGCAA
GATGAGTTTATTATTGAGTTATCTGAACGCTTAACTCGACAGCTCAGTATGTACAGTTCAGTTAATCAGCAACAAAAAAT
TGAGGGGCTTTGGTTGACTGGCGGGGGGGCAACCTTAGCGGCGATTGATGAGCACTTGTCCGAAAAACTGAATCTCCCCG
TTGAAATCCTAGACCCGTTAGCGCTATTGCAAAGCAAAGTGAAAAATAAAATTGACGCTGAGCCTTGCACTTTTAGTGTT
GCGGCCGGTTTAGCAATCCGAGGCATTCAGTGGCAAGGAGGGAAGCATGTTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

50.581

100

0.515
  pilM Vibrio campbellii strain DS40M4

46.176

100

0.464

Multiple sequence alignment