Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VCASEI_RS11970 Genome accession   NZ_AP018680
Coordinates   2552085..2553110 (-) Length   341 a.a.
NCBI ID   WP_086960399.1    Uniprot ID   -
Organism   Vibrio casei strain DSM 22364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2547085..2558110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCASEI_RS11945 aroK 2547759..2548277 (-) 519 WP_086960394.1 shikimate kinase AroK -
  VCASEI_RS11950 pilQ 2548528..2550363 (-) 1836 WP_089110418.1 type IV pilus secretin PilQ Machinery gene
  VCASEI_RS11955 pilP 2550400..2550912 (-) 513 WP_086960396.1 pilus assembly protein PilP Machinery gene
  VCASEI_RS11960 pilO 2550909..2551493 (-) 585 WP_086960397.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCASEI_RS11965 pilN 2551493..2552095 (-) 603 WP_086960398.1 PilN domain-containing protein Machinery gene
  VCASEI_RS11970 pilM 2552085..2553110 (-) 1026 WP_086960399.1 type IV pilus assembly protein PilM Machinery gene
  VCASEI_RS11975 - 2553282..2555846 (+) 2565 WP_086960400.1 penicillin-binding protein 1A -
  VCASEI_RS11980 oxyR 2555942..2556829 (-) 888 WP_089110417.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38071.84 Da        Isoelectric Point: 6.6978

>NTDB_id=70339 VCASEI_RS11970 WP_086960399.1 2552085..2553110(-) (pilM) [Vibrio casei strain DSM 22364]
MGKSLVTGIDIGHHSIKAVVMKPAGELFELVSYTEVSTTSAIFSENHTFDHHSIVKKLKELKKQLPIMARNVVLSVPDNA
VISKMLHIDSELDEREKEFAIYQVFGQQSPFPVEDLSLDYVKVEQETLQRVPTSTYQIYATRKEVIESRVDVVKKSGFKP
LVMDTQAHGLLRIWQLATQLNVEKKNWLLVDIGLTQTSICIIPQSRPAYYKDLSFGTQSMDVTSKPELALNQNNEMNIND
KSDEFISLLAERLTRQISLYGSVNQQQKIEGVWVTGGGACLLGMDEILSQGLGLPIEVLDPLSLLQGKYKKNKQSLEACT
FSVAAGLAIRGLQWQRGAHVA

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=70339 VCASEI_RS11970 WP_086960399.1 2552085..2553110(-) (pilM) [Vibrio casei strain DSM 22364]
ATGGGTAAATCTTTAGTCACGGGCATCGACATAGGTCACCACAGTATTAAAGCTGTAGTTATGAAACCTGCTGGAGAACT
GTTTGAGCTGGTGAGCTACACGGAAGTGTCAACAACCAGTGCTATTTTTTCAGAAAACCATACCTTTGATCATCATAGCA
TTGTAAAAAAACTTAAAGAGTTGAAAAAACAACTGCCCATAATGGCTCGTAATGTTGTGCTTTCTGTGCCAGATAATGCC
GTGATCAGTAAAATGTTGCATATAGATAGCGAACTCGATGAAAGAGAAAAAGAGTTTGCGATATATCAAGTGTTTGGTCA
GCAATCGCCATTTCCAGTTGAAGATCTTAGCCTTGATTACGTCAAAGTGGAGCAAGAAACATTGCAAAGAGTTCCCACCT
CGACTTATCAAATTTATGCCACTCGTAAAGAGGTAATTGAAAGCCGTGTCGATGTGGTAAAAAAATCAGGCTTTAAACCT
TTAGTGATGGATACCCAAGCTCATGGTTTATTACGCATATGGCAATTAGCGACGCAATTGAATGTGGAAAAGAAAAACTG
GCTATTGGTTGATATCGGCTTGACTCAAACGTCCATTTGCATCATTCCACAAAGTAGGCCTGCTTATTACAAAGATCTTT
CATTTGGCACCCAAAGCATGGATGTAACCTCTAAACCTGAACTTGCCTTAAATCAAAACAACGAGATGAATATCAATGAT
AAATCTGATGAATTTATTTCTTTGTTAGCAGAAAGACTCACCAGACAAATTAGTTTATATGGTTCGGTTAATCAGCAGCA
AAAAATTGAAGGTGTATGGGTGACTGGCGGTGGTGCTTGTTTGTTAGGGATGGACGAAATTCTATCACAAGGACTTGGAT
TACCTATCGAGGTGCTCGATCCATTGTCGTTATTACAAGGTAAATACAAAAAAAACAAACAGAGTCTTGAAGCATGCACT
TTTAGCGTGGCGGCTGGTTTGGCAATTCGTGGCCTTCAATGGCAAAGGGGTGCACATGTTGCATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-331)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

51.603

100

0.519
  pilM Vibrio campbellii strain DS40M4

42.815

100

0.428

Multiple sequence alignment