Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VCASEI_RS11960 Genome accession   NZ_AP018680
Coordinates   2550909..2551493 (-) Length   194 a.a.
NCBI ID   WP_086960397.1    Uniprot ID   -
Organism   Vibrio casei strain DSM 22364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2545909..2556493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCASEI_RS11940 aroB 2546644..2547729 (-) 1086 WP_086960393.1 3-dehydroquinate synthase -
  VCASEI_RS11945 aroK 2547759..2548277 (-) 519 WP_086960394.1 shikimate kinase AroK -
  VCASEI_RS11950 pilQ 2548528..2550363 (-) 1836 WP_089110418.1 type IV pilus secretin PilQ Machinery gene
  VCASEI_RS11955 pilP 2550400..2550912 (-) 513 WP_086960396.1 pilus assembly protein PilP Machinery gene
  VCASEI_RS11960 pilO 2550909..2551493 (-) 585 WP_086960397.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCASEI_RS11965 pilN 2551493..2552095 (-) 603 WP_086960398.1 PilN domain-containing protein Machinery gene
  VCASEI_RS11970 pilM 2552085..2553110 (-) 1026 WP_086960399.1 type IV pilus assembly protein PilM Machinery gene
  VCASEI_RS11975 - 2553282..2555846 (+) 2565 WP_086960400.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22393.78 Da        Isoelectric Point: 5.0424

>NTDB_id=70337 VCASEI_RS11960 WP_086960397.1 2550909..2551493(-) (pilO) [Vibrio casei strain DSM 22364]
MVDFRDLDFDDMPEWPVLPQVLAIGGLIIILQLVGIWFYLIPQHDELEQIHQQEVTLKSSIRIKAAKVAALPQLQAQLDE
LNTRYQYLLKQLPVQKELASMLSSVNDLGIKNDLNFTRVDWGGKQVQEFLYRLPLNIELTGAYDNIGQFSEAIAKLPRII
SLHDVDLQRVSQESSTLHFRVRAYTYQFKPEESK

Nucleotide


Download         Length: 585 bp        

>NTDB_id=70337 VCASEI_RS11960 WP_086960397.1 2550909..2551493(-) (pilO) [Vibrio casei strain DSM 22364]
ATGGTCGACTTTCGAGATTTAGATTTTGATGACATGCCTGAGTGGCCAGTATTACCACAAGTATTAGCAATTGGTGGGCT
TATTATTATTTTGCAATTGGTCGGTATTTGGTTCTATTTGATTCCTCAACATGACGAATTAGAGCAAATTCACCAGCAAG
AAGTGACCCTAAAAAGTTCTATACGTATTAAAGCTGCAAAAGTAGCGGCTTTACCTCAGTTACAAGCCCAATTAGATGAG
CTGAACACTCGTTATCAGTACTTGCTCAAGCAACTCCCCGTGCAGAAAGAACTGGCGAGCATGCTTTCTTCTGTTAATGA
TCTTGGTATTAAAAATGACTTGAATTTTACTCGTGTAGATTGGGGGGGAAAACAAGTACAAGAGTTTTTATATCGACTTC
CGTTAAATATTGAGCTGACTGGAGCCTATGACAACATAGGTCAGTTTTCGGAAGCTATTGCTAAGTTACCTCGAATCATC
AGTCTGCATGATGTCGATTTGCAACGTGTTAGCCAAGAAAGCAGTACTCTTCATTTTCGTGTGAGGGCTTATACCTATCA
ATTTAAGCCGGAGGAAAGCAAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

64.398

98.454

0.634
  pilO Vibrio campbellii strain DS40M4

63.158

97.938

0.619

Multiple sequence alignment