Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   VCASEI_RS11965 Genome accession   NZ_AP018680
Coordinates   2551493..2552095 (-) Length   200 a.a.
NCBI ID   WP_086960398.1    Uniprot ID   -
Organism   Vibrio casei strain DSM 22364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2546493..2557095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCASEI_RS11940 aroB 2546644..2547729 (-) 1086 WP_086960393.1 3-dehydroquinate synthase -
  VCASEI_RS11945 aroK 2547759..2548277 (-) 519 WP_086960394.1 shikimate kinase AroK -
  VCASEI_RS11950 pilQ 2548528..2550363 (-) 1836 WP_089110418.1 type IV pilus secretin PilQ Machinery gene
  VCASEI_RS11955 pilP 2550400..2550912 (-) 513 WP_086960396.1 pilus assembly protein PilP Machinery gene
  VCASEI_RS11960 pilO 2550909..2551493 (-) 585 WP_086960397.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCASEI_RS11965 pilN 2551493..2552095 (-) 603 WP_086960398.1 PilN domain-containing protein Machinery gene
  VCASEI_RS11970 pilM 2552085..2553110 (-) 1026 WP_086960399.1 type IV pilus assembly protein PilM Machinery gene
  VCASEI_RS11975 - 2553282..2555846 (+) 2565 WP_086960400.1 penicillin-binding protein 1A -
  VCASEI_RS11980 oxyR 2555942..2556829 (-) 888 WP_089110417.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 200 a.a.        Molecular weight: 23326.82 Da        Isoelectric Point: 9.5232

>NTDB_id=70338 VCASEI_RS11965 WP_086960398.1 2551493..2552095(-) (pilN) [Vibrio casei strain DSM 22364]
MLHDINLLPWREEKRTEYKQRFYGMVVLGVLVAISLQWFGSLYIDHLKTQQQSRNQELKTYIAELDKKLVNLKEIEAKHK
SILTRLRLVESLQNERNKTTDFMNLMPELVPEGVYVDKIKMNGREIEMSGVSDSTSRLATMLDNFESSSWLSDVEMHSII
SGKVLFGKKFQSFKLSFRFESEETERQQNKTVNTQAKGAK

Nucleotide


Download         Length: 603 bp        

>NTDB_id=70338 VCASEI_RS11965 WP_086960398.1 2551493..2552095(-) (pilN) [Vibrio casei strain DSM 22364]
ATGTTGCATGATATTAATTTATTACCTTGGCGTGAAGAAAAGCGTACGGAATACAAACAACGTTTCTATGGAATGGTCGT
GTTGGGGGTTCTGGTTGCGATTAGTTTGCAATGGTTTGGAAGTTTGTACATCGATCATTTAAAAACACAGCAGCAAAGTC
GTAATCAAGAGCTGAAAACGTATATTGCCGAGCTTGATAAAAAGCTCGTGAACCTTAAAGAAATTGAAGCGAAACATAAA
TCGATTCTTACTCGATTAAGGCTCGTTGAATCGTTACAAAATGAGCGTAATAAAACGACGGATTTTATGAATTTGATGCC
AGAACTTGTGCCTGAAGGTGTCTATGTCGACAAAATTAAAATGAATGGGCGAGAAATTGAAATGTCGGGTGTCAGTGACA
GTACGTCACGTTTAGCAACTATGCTGGATAATTTTGAAAGCTCATCTTGGTTATCCGATGTAGAAATGCATTCGATTATT
TCGGGAAAAGTATTGTTCGGTAAAAAATTCCAAAGTTTTAAATTGTCTTTCCGGTTTGAGTCTGAAGAAACGGAGCGTCA
ACAGAACAAAACAGTGAATACTCAAGCTAAGGGGGCCAAGTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

53.769

99.5

0.535
  pilN Vibrio campbellii strain DS40M4

57.222

90

0.515

Multiple sequence alignment