Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NGM44_RS04670 Genome accession   NZ_CP099960
Coordinates   971540..973192 (+) Length   550 a.a.
NCBI ID   WP_253224444.1    Uniprot ID   -
Organism   Moraxella sp. FZFQ2102     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 966540..978192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGM44_RS04640 (NGM44_04640) rimP 967833..968330 (-) 498 WP_253224441.1 ribosome maturation factor RimP -
  NGM44_RS04650 (NGM44_04650) - 969008..969985 (+) 978 WP_253224442.1 Fic family protein -
  NGM44_RS04660 (NGM44_04660) secG 970238..970558 (-) 321 WP_253224443.1 preprotein translocase subunit SecG -
  NGM44_RS04665 (NGM44_04665) tpiA 970607..971362 (-) 756 WP_253224628.1 triose-phosphate isomerase -
  NGM44_RS04670 (NGM44_04670) pilB 971540..973192 (+) 1653 WP_253224444.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NGM44_RS04675 (NGM44_04675) - 973197..974417 (+) 1221 WP_253224445.1 type II secretion system F family protein -
  NGM44_RS04680 (NGM44_04680) - 974417..975274 (+) 858 WP_253224446.1 A24 family peptidase -
  NGM44_RS04685 (NGM44_04685) coaE 975316..975942 (+) 627 WP_253224447.1 dephospho-CoA kinase -
  NGM44_RS04690 (NGM44_04690) - 976183..977475 (+) 1293 WP_253224448.1 alpha-hydroxy-acid oxidizing protein -

Sequence

Protein


Download         Length: 550 a.a.        Molecular weight: 60326.97 Da        Isoelectric Point: 6.3241

>NTDB_id=702979 NGM44_RS04670 WP_253224444.1 971540..973192(+) (pilB) [Moraxella sp. FZFQ2102]
MLTAFHHRLIAQLSQLGCDADALTTAMTDAYANSTPWTDAVINAQLVSPKPLGDTMAILLNMPLVDLDRHDTRDAGTLID
PRLIARHRVLPLSKDNSTLTLAMSDPTDRDAIQSIRFHTHLNVTPVLVEADKLSRHLDKLSLGAPVVLDEHLDDEDIEDD
PIVDAPTVQFVQKILADAVQLGASDIHFEPYENSFRVRYRIDGVMSSVNTPPKSQAGKITTRLKVMAHLDIAERRKPQDG
RIKFRLSEHKSVDFRVSTLPTLFGEKVVLRILDSAQTLIGMDALGLNATQKQQFTDALHKPQGMILITGPTGSGKTISLY
TALGLLNRGDNNILTAEDPVEINLDGINQTNINPKIGLDFADLLRAFLRQDPDVIMVGEIRDTETAEIAIKAAQTGHLVL
STLHTNSAAATLTRLGSMGVPSFQLASGVNLIIAQRLARRLCDDCKRAVVLPPQVLREAGFGDDDISRTHQLYEPVGCHR
CRDGYRGRVGIFELLPITPNIRKLIFDRADSDVIDHANRQAGNISLRQSGIAAVMAGITSLTEMHRVTSD

Nucleotide


Download         Length: 1653 bp        

>NTDB_id=702979 NGM44_RS04670 WP_253224444.1 971540..973192(+) (pilB) [Moraxella sp. FZFQ2102]
ATGCTGACCGCGTTTCATCATCGACTCATTGCACAGTTATCACAGCTTGGTTGTGATGCTGACGCGCTCACCACCGCCAT
GACTGATGCATATGCCAACTCTACCCCATGGACAGATGCTGTAATCAATGCACAGCTTGTCAGTCCAAAGCCGCTTGGTG
ATACAATGGCGATACTGCTCAATATGCCGCTTGTCGATTTAGATCGTCATGATACGCGCGATGCTGGCACGCTGATCGAT
CCCAGGCTCATTGCGCGCCATCGCGTCTTGCCACTTAGCAAGGATAATAGCACGCTGACCCTTGCCATGAGCGATCCGAC
TGACCGCGATGCCATTCAGTCCATTCGCTTTCACACACATCTGAATGTCACGCCAGTACTGGTTGAAGCTGATAAATTGT
CGCGACATCTTGATAAGCTGAGTCTGGGCGCGCCCGTGGTGCTCGATGAACATCTGGATGATGAAGACATCGAAGACGAT
CCCATCGTTGATGCGCCGACGGTACAATTTGTCCAAAAAATCCTAGCCGACGCGGTGCAACTGGGCGCATCTGACATTCA
TTTTGAGCCTTATGAAAACAGCTTTCGCGTGCGCTATCGCATCGATGGCGTGATGAGCAGTGTCAATACACCACCCAAAA
GCCAAGCGGGTAAAATCACCACGCGGCTTAAAGTCATGGCGCATTTGGACATCGCCGAGCGACGCAAACCCCAAGATGGG
CGTATCAAATTTCGCTTATCTGAACATAAATCGGTGGATTTTCGCGTCAGTACTTTGCCTACTTTATTCGGTGAAAAAGT
CGTCTTACGAATCTTGGACTCAGCGCAGACGCTCATCGGCATGGACGCGCTAGGGCTTAATGCCACACAAAAACAGCAGT
TCACCGACGCCCTGCACAAGCCCCAAGGCATGATTTTGATCACAGGTCCGACAGGCAGCGGCAAGACCATCTCGCTATAT
ACCGCGCTTGGGTTATTAAACCGTGGCGATAATAATATCTTGACCGCCGAAGATCCTGTGGAAATCAATCTTGACGGCAT
TAACCAGACTAACATCAATCCAAAAATCGGCTTGGATTTTGCCGACTTATTACGCGCGTTCTTACGCCAAGATCCTGATG
TGATCATGGTCGGTGAAATTCGCGATACCGAGACGGCTGAGATCGCGATCAAGGCAGCACAAACAGGGCATTTGGTGCTA
TCGACCCTGCATACCAATTCTGCCGCTGCCACCTTGACGCGTCTTGGCAGTATGGGCGTGCCAAGCTTTCAACTTGCCAG
TGGCGTCAATCTGATCATCGCCCAACGCCTAGCGCGGCGGCTGTGTGATGACTGCAAGCGCGCGGTAGTGCTACCGCCAC
AGGTGCTGCGTGAAGCAGGCTTTGGTGATGATGACATCTCACGCACCCATCAGCTGTACGAGCCTGTGGGCTGCCATCGC
TGCCGTGATGGCTATCGTGGGCGTGTGGGTATCTTTGAGCTGCTGCCGATCACGCCCAATATTCGCAAGTTGATTTTTGA
TCGTGCTGACAGCGATGTCATTGATCATGCCAATCGCCAAGCAGGTAATATCAGCTTACGCCAATCAGGAATCGCCGCTG
TCATGGCAGGCATCACAAGCTTGACTGAAATGCACCGCGTGACATCGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-140)

Type II/IV secretion system protein (T2SSE)

(173-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.064

100

0.524
  pilB Acinetobacter baylyi ADP1

49.65

100

0.516
  pilB Legionella pneumophila strain ERS1305867

50.275

99.091

0.498
  pilB Vibrio cholerae strain A1552

47.32

98.364

0.465
  pilF Neisseria gonorrhoeae MS11

47.048

95.455

0.449
  pilB Vibrio campbellii strain DS40M4

46.082

97.455

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.693

97.091

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.5

100

0.376
  pilF Thermus thermophilus HB27

38.269

94.545

0.362