Detailed information NTG0702122

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PCP81_RS25100
• Genome accession: NZ_CP115288
• Coordinates: 5380629..5381693 (+)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain F005
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5375629..5386693

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP81_RS25090 (PCP81_25085)	-	5376600..5377868 (+)	1269	WP_004347579.1	malic enzyme-like NAD(P)-binding protein	-
PCP81_RS25095 (PCP81_25090)	-	5377973..5380444 (-)	2472	WP_003095841.1	penicillin-binding protein 1A	-
PCP81_RS25100 (PCP81_25095)	pilM	5380629..5381693 (+)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
PCP81_RS25105 (PCP81_25100)	pilN	5381693..5382289 (+)	597	WP_003116134.1	type 4a pilus biogenesis protein PilN	-
PCP81_RS25110 (PCP81_25105)	pilO	5382286..5382909 (+)	624	WP_003116135.1	type 4a pilus biogenesis protein PilO	Machinery gene
PCP81_RS25115 (PCP81_25110)	comL/pilP	5382906..5383430 (+)	525	WP_003116136.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
PCP81_RS25120 (PCP81_25115)	pilQ	5383484..5385616 (+)	2133	WP_003116137.1	type 4a pilus secretin PilQ	Machinery gene
PCP81_RS25125 (PCP81_25120)	aroK	5385628..5386146 (+)	519	WP_003095833.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=702122 PCP81_RS25100 WP_003110888.1 5380629..5381693(+) (pilM) [Pseudomonas aeruginosa strain F005]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=702122 PCP81_RS25100 WP_003110888.1 5380629..5381693(+) (pilM) [Pseudomonas aeruginosa strain F005]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466