Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NH234_RS02950 Genome accession   NZ_CP099760
Coordinates   657027..658091 (+) Length   354 a.a.
NCBI ID   WP_085732569.1    Uniprot ID   -
Organism   Pseudomonas sp. stari2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 652027..663091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH234_RS02940 (NH234_02935) - 652929..654197 (+) 1269 WP_085711053.1 malic enzyme-like NAD(P)-binding protein -
  NH234_RS02945 (NH234_02940) - 654363..656810 (-) 2448 WP_085732568.1 penicillin-binding protein 1A -
  NH234_RS02950 (NH234_02945) pilM 657027..658091 (+) 1065 WP_085732569.1 pilus assembly protein PilM Machinery gene
  NH234_RS02955 (NH234_02950) - 658091..658657 (+) 567 WP_085732570.1 PilN domain-containing protein -
  NH234_RS02960 (NH234_02955) pilO 658654..659277 (+) 624 WP_085732571.1 type 4a pilus biogenesis protein PilO -
  NH234_RS02965 (NH234_02960) - 659274..659798 (+) 525 WP_085732572.1 pilus assembly protein PilP -
  NH234_RS02970 (NH234_02965) pilQ 659812..661890 (+) 2079 WP_367255593.1 type IV pilus secretin PilQ Machinery gene
  NH234_RS02975 (NH234_02970) aroK 661895..662413 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38180.74 Da        Isoelectric Point: 4.7582

>NTDB_id=701556 NH234_RS02950 WP_085732569.1 657027..658091(+) (pilM) [Pseudomonas sp. stari2]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGISPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLGTPTQVANP
FFDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=701556 NH234_RS02950 WP_085732569.1 657027..658091(+) (pilM) [Pseudomonas sp. stari2]
GTGCTAGGACTCTTCAACAAAAAGACCAATACGTTACTGGGGATCGACATCAGTTCCACTTCGGTGAAGCTGCTGGAGCT
GAGCCGTCAGGGTGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCCAACGCCGTGGTCGAGAAAAACA
TCGCCGAACTCGAAGGCGTCGGGCATGCCCTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCGGCGGTGATCACTAAGGTCATCGAAATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGTTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGTATCTCGCCGC
GCAATCCCGAGCGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGTGAGGCTGCACTGGCCCTC
GCCGGCCTGACTGCGCGGGTGGTGGATGTCGAGGCCTATGCGCTTGAGCGCTCGTTCGGCCTGCTGGCCACGCAACTGGC
GGCTTCCCAGGAGCGCTTGACCGTTGCGGTGGTCGATATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTATACCCGCGAGCAACTCTTTGGCGGGCGCCAGCTGACCGAGGAAATCCAGCGCCGTTATGGCCTGACCATT
GAGCAGGCCGGGCTGGCCAAGAAGCAGGGCGGTCTGCCGGACGATTACGTCAGCGAGGTCCTGCAGCCGTTTCGCGAGGC
GCTGGTGCAGCAGGTCTCACGCTCCTTGCAGTTTTTCTTCGCTTCCGGCCAGTACAACGCGGTCGACCATATTTTGCTGG
CCGGCGGCACGGCGTCGGTGCCCGGGCTGGACCGGCTGATTGAAGAACGCCTGGGGACGCCGACCCAGGTTGCCAATCCG
TTTTTCGACATGGCCTTGAGCAGCAAAGTCAATGCCGGGGCCCTGGCCAGTGATGCGCCGGCCCTGATGATTGCCTGTGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486