Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NH234_RS02970 Genome accession   NZ_CP099760
Coordinates   659812..661890 (+) Length   692 a.a.
NCBI ID   WP_367255593.1    Uniprot ID   -
Organism   Pseudomonas sp. stari2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 654812..666890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH234_RS02950 (NH234_02945) pilM 657027..658091 (+) 1065 WP_085732569.1 pilus assembly protein PilM Machinery gene
  NH234_RS02955 (NH234_02950) - 658091..658657 (+) 567 WP_085732570.1 PilN domain-containing protein -
  NH234_RS02960 (NH234_02955) pilO 658654..659277 (+) 624 WP_085732571.1 type 4a pilus biogenesis protein PilO -
  NH234_RS02965 (NH234_02960) - 659274..659798 (+) 525 WP_085732572.1 pilus assembly protein PilP -
  NH234_RS02970 (NH234_02965) pilQ 659812..661890 (+) 2079 WP_367255593.1 type IV pilus secretin PilQ Machinery gene
  NH234_RS02975 (NH234_02970) aroK 661895..662413 (+) 519 WP_011332081.1 shikimate kinase AroK -
  NH234_RS02980 (NH234_02975) aroB 662555..663655 (+) 1101 WP_119429530.1 3-dehydroquinate synthase -
  NH234_RS02985 (NH234_02980) - 663666..665264 (+) 1599 WP_085732575.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75208.43 Da        Isoelectric Point: 5.4082

>NTDB_id=701557 NH234_RS02970 WP_367255593.1 659812..661890(+) (pilQ) [Pseudomonas sp. stari2]
MNRIFSTLGFSLWIALLSPMVQAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVVSQLASKTRD
LGSGNARTATVVEANDRTRLIINLTQLAPYDTRVEGNTLFVVVGQGSKVTAPRPATKAPRATPVAAPVRTFAPTARAIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPLTVDDLQKRNADRNTYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=701557 NH234_RS02970 WP_367255593.1 659812..661890(+) (pilQ) [Pseudomonas sp. stari2]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTGTCGCCGATGGTTCAAGCGGCAAATCTGAA
GACGCTGGACGTGGCGGCGCTGCCGGGCGATCGCGTCGAGTTGAAGCTATCGTTCGACGGCCCACCGCCACAGCCCAAGG
GCTACACCACCGATTCGCCGGCGCGGATCGCTCTGGATCTGCCGGGAGTGGTCAGCCAGCTCGCGAGCAAGACTCGTGAC
CTCGGTAGCGGTAATGCGCGCACTGCCACAGTGGTCGAGGCCAACGACCGTACCCGGTTGATCATCAATCTGACGCAACT
GGCGCCGTATGACACCCGGGTCGAGGGGAATACGTTGTTCGTGGTGGTCGGGCAAGGTTCCAAAGTCACCGCGCCGCGAC
CTGCCACCAAGGCTCCACGTGCGACACCGGTGGCGGCACCGGTCCGGACGTTCGCGCCGACCGCCAGGGCCATCCGAGGC
GTGGACTTCCAGCGCGGCACGGCGGGCGAGGGCAATGTCGTCATCGACCTGTCCGACCCGACCATCGCGCCGGACATCCA
GGAACATGACGGCAAGATCATTCTCAATTTCGCTCGCACCCAACTGCCCGAACCGTTGCGGGTGCGTCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCTGGTGTCAGCGGTGATCGAGCGACCATCACCGTCGAGCCCAGCGGCACG
TTCGACTATTCCACTTACCAGACCGACAACAAGCTGACCGTGAGCATCCGGCCGTTGACCGTCGATGACCTGCAAAAGCG
CAATGCTGACCGCAACACTTATAGCGGCGAAAAGCTTTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGCAAC
TGATCGCCGATTTCACCAACCTCAATCTGGTCGCCAGCGACACGGTGCAGGGCGGAATCACCCTGCGTCTGCAAAACGTG
CCGTGGGATCAGGCGCTGGATCTAGTGCTCAAGACCAAAGGCCTGGACAAGCGCAAAGTCGGAAATGTGTTGCTGGTGGC
GCCGGCGGATGAAATCGCCGCCCGCGAACGGCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCGCTGCGCC
GTGAACTGTTGCAAGTGAATTACGCCAAGGCCGCGGATATCGCCAAGTTGTTCCAGTCGGTCACCAGCGCCGAGGCCAAA
GTCGACGAACGGGGTTCGATCACCGTCGATGAGCGCACCAACAACATCATCGCCTACCAGACCCAGGATCGTCTCGACGA
GCTGCGGCGGATCGTGGCGCAACTGGATATCCCGGTGCGTCAGGTGATGATCGAGGCACGGATCGTCGAGGCCAACGTCG
ATTACGACAAGAGCCTGGGCGTTCGCTGGGGCGGATCGATCCAGAACAAGGGCAATTGGAATACCTCCGGGGTCAGCAAC
GGCTCGTCGACCACCATTGGTACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGTACAGTCAACAACAC
CTCCGGGATCGGCATTGCGTTCATCACCGACAACGTCTTGCTGGACCTTGAGCTGACGGCGATGGAGAAGACCGGCAACG
GGGAGATCGTCTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCTACATCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAGGTCACGCCGCAGATCAC
CCCGGACAATCGCATCATCATGGAGGTGAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAAAACGAGGTCAATGCCAAGGTACTTGTGAACGACGGCGAGACCATCGTGATTGGCGGGGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTAGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.028

100

0.744
  comQ Acinetobacter baylyi ADP1

41.066

100

0.412
  pilQ Acinetobacter baumannii D1279779

37.917

100

0.41
  pilQ Acinetobacter baumannii strain A118

37.917

100

0.41
  pilQ Legionella pneumophila strain Lp02

38.074

97.543

0.371
  pilQ Legionella pneumophila strain ERS1305867

38.039

97.254

0.37