Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NH461_RS02005 Genome accession   NZ_CP099734
Coordinates   410576..412381 (-) Length   601 a.a.
NCBI ID   WP_315903232.1    Uniprot ID   -
Organism   Photobacterium sp. TY1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 405576..417381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH461_RS01975 (NH461_01970) mutT 406227..406625 (+) 399 WP_261601675.1 8-oxo-dGTP diphosphatase MutT -
  NH461_RS01980 (NH461_01975) yacG 406739..406954 (-) 216 WP_261601676.1 DNA gyrase inhibitor YacG -
  NH461_RS01985 (NH461_01980) zapD 406962..407699 (-) 738 WP_261601677.1 cell division protein ZapD -
  NH461_RS01990 (NH461_01985) coaE 407728..408333 (-) 606 WP_261601678.1 dephospho-CoA kinase -
  NH461_RS01995 (NH461_01990) pilD 408336..409205 (-) 870 WP_261601679.1 prepilin peptidase Machinery gene
  NH461_RS02000 (NH461_01995) pilC 409327..410547 (-) 1221 WP_261601680.1 type II secretion system F family protein Machinery gene
  NH461_RS02005 (NH461_02000) pilB 410576..412381 (-) 1806 WP_315903232.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NH461_RS02010 (NH461_02005) - 412385..412786 (-) 402 WP_315903233.1 pilin -
  NH461_RS02020 (NH461_02015) nadC 413125..414012 (-) 888 WP_261602808.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NH461_RS02025 (NH461_02020) ampD 414278..414832 (+) 555 WP_261601681.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NH461_RS02030 (NH461_02025) pdhR 415123..415896 (+) 774 WP_261601682.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 66366.53 Da        Isoelectric Point: 6.4632

>NTDB_id=701387 NH461_RS02005 WP_315903232.1 410576..412381(-) (pilB) [Photobacterium sp. TY1-4]
MNTHLSSTLHQAGLLSPVQRQQVVDTVQADGIPVPSALVQLGLFTSAELARQLEHLFAIALVDVHQYDYTASCHSLNQRE
LILRHRALPLSQNDTTLYLGLSDPTQMDALDEFRFVTGKTIEPLLLECKQLDSAIRRLYGSEIGETSHQRSLSDHDLGEL
VNLSEGEFEETATDLSQDSAPVTRYINQVLLDAVRKHASDIHFEPYEETYRIRFRCDGILHQHATPPAHLSRRLSTRLKV
MSKLNIAERRQPQDGRIKLRLSPTLAIDLRVSTLPTLWGEKVVLRILDGTSAKLDIDLLGYTPQQKQAYLEALQKPQGMI
LMTGPTGSGKTVSLYTGLNILNTEARNISTAEDPVEINLPGVNQVQINPTVGLGFAEALRSFLRQDPDIVMVGEIRDQET
GSIAVKAAQTGHLVLSTLHTNSAAETITRLTHMGLETFNLASSLSLIIAQRLARRLCKHCKQPDPLDPLTREALAIAPGA
SLFKANEQGCDECNKGYLGRIGIYEVMPFSRSLAQALMAGASTLELEDIARREGMQTLQQSGIETLEAGITSLKELQRVL
QISVTSPTVTPAQQQRAQRQTRQTNEPRVHQVSPALSHAES

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=701387 NH461_RS02005 WP_315903232.1 410576..412381(-) (pilB) [Photobacterium sp. TY1-4]
ATGAACACCCACCTCTCTTCTACCCTGCACCAGGCCGGGCTGCTCAGCCCGGTGCAGCGGCAACAAGTGGTGGATACAGT
CCAGGCCGACGGCATTCCGGTGCCGTCGGCCCTGGTCCAGCTCGGGCTGTTCACCAGCGCGGAGCTGGCCCGCCAGCTTG
AGCACCTGTTTGCCATCGCCCTGGTTGATGTCCACCAGTACGACTACACTGCCAGCTGCCACAGCCTGAACCAGCGCGAG
CTGATCCTGCGCCACCGGGCACTGCCACTGAGCCAGAATGACACCACCCTGTATCTCGGCCTGAGCGATCCGACCCAAAT
GGATGCCCTGGATGAGTTCCGCTTTGTCACCGGTAAAACTATTGAGCCGCTACTGCTCGAGTGCAAGCAGCTCGACAGCG
CGATCCGGCGCCTGTACGGCAGTGAAATCGGCGAAACCAGCCACCAGCGCAGCCTCAGTGACCACGATCTCGGTGAGCTG
GTCAATTTGAGCGAAGGCGAGTTTGAAGAGACCGCCACCGACCTGAGCCAGGACAGCGCCCCGGTCACCCGCTACATCAA
CCAGGTATTGCTGGACGCCGTGCGCAAGCACGCCTCGGACATTCACTTTGAACCTTACGAGGAAACCTACCGCATCCGCT
TTCGCTGTGACGGGATCCTGCACCAGCATGCCACGCCGCCGGCACACCTGTCGCGCCGCCTGTCAACCCGGCTCAAAGTG
ATGTCCAAACTCAATATTGCCGAACGGCGCCAGCCCCAGGACGGACGAATTAAACTCCGGCTCTCGCCGACCCTGGCAAT
CGATCTGCGTGTTTCGACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGCTACGGATCCTTGACGGTACCAGTGCCAAGC
TCGATATTGATCTACTCGGCTACACCCCGCAGCAAAAACAGGCATATCTGGAAGCGCTGCAAAAACCCCAGGGAATGATC
CTGATGACCGGGCCGACCGGCAGCGGTAAAACGGTGTCGCTGTACACCGGGCTGAACATTCTCAACACCGAGGCGCGCAA
TATTTCCACCGCCGAAGATCCGGTCGAGATCAACCTGCCCGGGGTCAACCAGGTCCAGATCAACCCCACCGTCGGGCTGG
GGTTTGCCGAAGCCCTGCGCTCCTTTCTGCGCCAGGATCCGGACATTGTGATGGTCGGGGAGATCCGGGATCAGGAAACC
GGCTCAATCGCAGTCAAAGCCGCCCAAACCGGCCACCTGGTGCTGTCCACTCTGCACACCAACTCAGCGGCGGAAACCAT
CACCCGCCTGACGCACATGGGCCTGGAGACCTTCAACCTGGCCTCGTCCCTGAGCCTGATCATCGCCCAGCGTCTGGCAC
GCCGGCTATGCAAACACTGCAAACAGCCCGATCCGCTTGACCCGCTGACCCGGGAGGCGCTGGCCATTGCGCCGGGAGCC
AGCTTATTCAAAGCCAACGAGCAAGGCTGCGATGAGTGCAACAAGGGCTATCTGGGCCGGATCGGCATTTACGAAGTCAT
GCCCTTCAGCCGCTCGTTAGCCCAGGCACTGATGGCCGGCGCGTCTACGCTGGAGCTGGAAGATATTGCCCGCCGTGAAG
GGATGCAAACCCTGCAGCAATCCGGGATCGAAACCCTTGAGGCCGGGATCACCAGCCTCAAAGAGCTGCAGCGGGTACTG
CAAATCAGCGTTACATCCCCGACGGTCACCCCAGCACAGCAACAACGAGCGCAGCGGCAGACACGGCAAACCAACGAGCC
CCGGGTTCACCAGGTGTCCCCGGCCCTATCGCATGCCGAATCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.389

93.344

0.582
  pilB Vibrio parahaemolyticus RIMD 2210633

61.497

93.344

0.574
  pilB Vibrio campbellii strain DS40M4

61.032

93.511

0.571
  pilB Legionella pneumophila strain ERS1305867

50.092

90.682

0.454
  pilB Acinetobacter baumannii D1279779

51.83

86.356

0.448
  pilB Acinetobacter baylyi ADP1

51.765

84.859

0.439
  pilF Neisseria gonorrhoeae MS11

44.286

93.178

0.413