Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NH461_RS01165 Genome accession   NZ_CP099734
Coordinates   250095..251816 (+) Length   573 a.a.
NCBI ID   WP_410000102.1    Uniprot ID   -
Organism   Photobacterium sp. TY1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 245095..256816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH461_RS01145 (NH461_01140) pilM 247358..248362 (+) 1005 WP_261601544.1 type IV pilus biogenesis protein PilM -
  NH461_RS01150 (NH461_01145) - 248359..248949 (+) 591 WP_261601545.1 PilN domain-containing protein -
  NH461_RS01155 (NH461_01150) - 248946..249539 (+) 594 WP_261601546.1 type 4a pilus biogenesis protein PilO -
  NH461_RS01160 (NH461_01155) - 249541..250071 (+) 531 WP_261601547.1 pilus assembly protein PilP -
  NH461_RS01165 (NH461_01160) pilQ 250095..251816 (+) 1722 WP_410000102.1 type IV pilus secretin PilQ Machinery gene
  NH461_RS01170 (NH461_01165) aroK 252008..252526 (+) 519 WP_261601548.1 shikimate kinase AroK -
  NH461_RS01175 (NH461_01170) aroB 252551..253630 (+) 1080 WP_261601549.1 3-dehydroquinate synthase -
  NH461_RS01180 (NH461_01175) - 253645..255279 (+) 1635 WP_261601550.1 AAA family ATPase -
  NH461_RS01185 (NH461_01180) - 255388..256206 (+) 819 WP_261601551.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  NH461_RS01190 (NH461_01185) - 256248..256472 (-) 225 WP_261601552.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62504.88 Da        Isoelectric Point: 4.8261

>NTDB_id=701382 NH461_RS01165 WP_410000102.1 250095..251816(+) (pilQ) [Photobacterium sp. TY1-4]
MKRDLSMKRAGCWRWDTAGVWAAGMLLWLVAASASAIEVVDNKILDIDFRRDGDGRGIVSVDLAKTRALVDVRQVGRQLY
VELVDAAIDDDMLYVLDVADFATVVTSVETLRGEKGTRLKLSMSDGFHYDYRLEGTRLSVMVTALPTEASSGQISYSGKP
ISINFQDIPVRNVLQLIAEYNDFNLVVSDSVTGTVTLRLDNVPWPQVLDIILQAKGLDKRVKDSVVLIAPKQELALSEQQ
ALEAKQQALALATLRSDIVQVHYAKATELAELLRGKEEGSSMLSERGSLHVDERTNALIIHDLGERIDKIKAIVAALDIP
VKQVHIEARIVSVNEDDVDEIGVRWGVLGSNGDTTVGGSIESNLVSSGLLESSDTPVTDFLNVNLGATEPGAARVAFQVA
KMGDILLDLELSALQSEKKLEIISSPRLVTTDKKMAYIEQGTEIPYLEASSSGAVSVGFKKAVLSLMVTPQITPDNKLVL
DLMVTQDKKGSEVKTGTGEAVAIDTQRIGTQVLVENGETVVLGGIYQHQINNIMRKVPLLGDLPVLGALFRHKLEQFTKR
ELLIFVTPKIVMQ

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=701382 NH461_RS01165 WP_410000102.1 250095..251816(+) (pilQ) [Photobacterium sp. TY1-4]
ATGAAGCGCGATTTGAGCATGAAAAGGGCGGGATGCTGGCGTTGGGACACCGCCGGAGTATGGGCGGCCGGGATGTTGCT
GTGGCTGGTGGCGGCATCCGCGTCTGCCATTGAGGTGGTGGACAATAAGATCCTGGATATTGATTTCCGGCGCGATGGCG
ACGGACGGGGGATAGTCAGTGTTGATCTGGCCAAAACCCGGGCGCTGGTCGATGTCCGCCAGGTCGGACGGCAGCTGTAT
GTTGAGCTGGTCGATGCGGCGATTGACGATGACATGCTGTATGTCCTGGATGTCGCGGACTTTGCGACTGTGGTGACATC
GGTGGAGACGCTGCGCGGGGAGAAAGGCACCCGGCTGAAACTGTCGATGAGCGACGGTTTCCACTATGACTATCGCCTGG
AAGGGACGCGGCTCAGTGTGATGGTGACCGCGCTGCCGACGGAAGCGTCGTCCGGGCAAATCAGCTATAGCGGCAAGCCG
ATTTCAATTAACTTTCAGGATATACCCGTTCGAAACGTCTTGCAGTTGATCGCCGAGTATAACGATTTCAACCTGGTGGT
CTCCGACAGTGTCACCGGCACGGTGACCTTGCGGCTCGACAATGTGCCCTGGCCGCAGGTGCTGGATATCATTTTGCAGG
CCAAGGGGCTGGATAAGCGGGTCAAAGACTCGGTGGTGCTGATTGCGCCGAAGCAGGAGCTGGCTTTGAGTGAACAGCAG
GCGCTGGAAGCGAAGCAACAGGCACTGGCACTGGCGACGCTGCGTTCGGACATTGTTCAGGTGCACTATGCCAAAGCGAC
GGAGCTGGCCGAGTTGTTGCGGGGCAAGGAAGAGGGTTCCAGTATGCTGTCAGAGCGGGGCTCACTGCATGTGGACGAGC
GGACGAATGCGTTGATCATTCATGACTTGGGTGAGCGTATCGACAAAATCAAAGCCATCGTGGCCGCGCTGGATATCCCG
GTGAAGCAGGTGCATATCGAAGCCCGGATTGTGTCGGTCAATGAAGATGATGTCGACGAGATCGGGGTACGGTGGGGCGT
GTTGGGCAGCAATGGCGACACCACTGTCGGCGGCTCGATTGAGAGTAATCTCGTTTCGTCCGGCCTGCTGGAGAGCAGCG
ATACACCCGTGACTGATTTTCTCAATGTGAATCTGGGGGCCACCGAGCCCGGGGCGGCGCGGGTCGCTTTTCAGGTGGCG
AAGATGGGCGATATTTTGCTGGATCTCGAGCTCTCGGCCCTGCAGTCGGAGAAAAAGCTGGAAATTATCTCCAGCCCCCG
CCTGGTGACCACGGACAAAAAGATGGCGTATATCGAACAGGGCACCGAAATCCCGTATCTCGAGGCGTCCTCCAGCGGTG
CGGTCTCGGTCGGGTTTAAAAAGGCCGTCCTGAGCCTGATGGTCACGCCGCAAATCACACCGGATAATAAACTGGTGCTG
GATCTGATGGTGACCCAGGACAAGAAGGGCAGCGAGGTGAAGACCGGCACTGGGGAGGCGGTTGCCATTGATACCCAGCG
GATCGGCACCCAGGTGCTGGTCGAAAATGGCGAGACCGTGGTGCTCGGTGGGATTTACCAGCACCAGATCAATAACATCA
TGCGCAAGGTCCCGTTATTGGGGGACTTACCGGTGCTGGGGGCGCTGTTCCGCCATAAGCTGGAGCAGTTTACCAAGCGT
GAGCTGTTGATCTTTGTGACTCCGAAAATCGTCATGCAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(183-231)

Bacterial type II/III secretion system short domain (Secretin_N)

(258-323)

Bacterial type II and III secretion system protein (Secretin)

(413-571)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

58.722

100

0.593
  pilQ Vibrio cholerae strain A1552

58.722

100

0.593
  pilQ Vibrio campbellii strain DS40M4

60.36

96.859

0.585
  pilQ Legionella pneumophila strain Lp02

43.173

94.59

0.408
  pilQ Legionella pneumophila strain ERS1305867

43.173

94.59

0.408
  comQ Acinetobacter baylyi ADP1

39.931

100

0.401
  pilQ Pseudomonas aeruginosa PAK

40.399

96.335

0.389
  pilQ Acinetobacter baumannii D1279779

37.197

100

0.375
  pilQ Acinetobacter baumannii strain A118

37.197

100

0.375