Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NF672_RS22280 Genome accession   NZ_CP099608
Coordinates   5034853..5036553 (+) Length   566 a.a.
NCBI ID   WP_252873379.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain OTU5BARRA1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5029853..5041553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF672_RS22260 (NF672_22260) - 5030021..5032972 (+) 2952 WP_252873378.1 DUF748 domain-containing protein -
  NF672_RS22265 (NF672_22265) - 5032969..5033268 (+) 300 WP_064364128.1 DUF2845 domain-containing protein -
  NF672_RS22270 (NF672_22270) - 5033536..5033889 (-) 354 WP_064364127.1 BON domain-containing protein -
  NF672_RS22275 (NF672_22275) - 5034189..5034626 (-) 438 WP_064364126.1 pilin -
  NF672_RS22280 (NF672_22280) pilB 5034853..5036553 (+) 1701 WP_252873379.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF672_RS22285 (NF672_22285) pilC 5036556..5037773 (+) 1218 WP_065615667.1 type II secretion system F family protein Machinery gene
  NF672_RS22290 (NF672_22290) pilD 5037775..5038647 (+) 873 WP_252873380.1 A24 family peptidase Machinery gene
  NF672_RS22295 (NF672_22295) coaE 5038644..5039267 (+) 624 WP_042610481.1 dephospho-CoA kinase -
  NF672_RS22300 (NF672_22300) yacG 5039264..5039464 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF672_RS22305 (NF672_22305) - 5039514..5040203 (-) 690 WP_065615664.1 energy-coupling factor ABC transporter permease -
  NF672_RS22310 (NF672_22310) - 5040393..5041019 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF672_RS22315 (NF672_22315) - 5041016..5041543 (+) 528 WP_065615663.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62596.65 Da        Isoelectric Point: 6.1674

>NTDB_id=701051 NF672_RS22280 WP_252873379.1 5034853..5036553(+) (pilB) [Pseudomonas moraviensis strain OTU5BARRA1]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMANVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=701051 NF672_RS22280 WP_252873379.1 5034853..5036553(+) (pilB) [Pseudomonas moraviensis strain OTU5BARRA1]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAATTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGTGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAGATCG
CCGAGATCGCCTCCGAACATTTCGGCATGGCGCTGCTCGATCTCAATTGCCTCGACAAGGAGACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTTCGCCAGCACTGCGCCCTGCCCTTGTGGCGGCGTGGCAACAAATTGTTCGTGGGTCTATCCGA
CCCGAGCAATCAGCAGGCAATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTCGAGGACGACA
AGCTCAGCGACGCCATCGACAAGTTCTTCGACAGCCATGCTTCGGGCCTGGAAGAAATGGCCAATGTCGATCTGGACGGC
CTCGACGTCGAGTCCATCGATGACAACAAGCAGGACGTTATCGGCGGCCTCGATGCGGACGATGCTCCAGTCGTGCGCTT
CGTGCACAAGATGCTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAGAAAAACTACCGGG
TACGCATGCGCACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATCCAGTTGGCCGGGCGCATCGCCGCACGTTTG
AAGGTCATGGCCAGCCTCGATATTTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTTTCGAAAAGCAA
ATCCATCGATTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CGCAAATGGGCATCGACGCCTTGGGTTACGAGGCCGAACAAAAAGCCTTGTACCTCGCTGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACCGGCCCCACCGGTTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCCGCCAGG
GGCTGGATTTCGCTCAGGCCCTGCGTTCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGTCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCGGCGGA
AACCCTCACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAATTGTGCGCGCACTGCAAGCGGCCGCTGGAGATCCCACACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGTCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAAAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAGATCGACATCCAGA
TGCGCCGCGACGGTTTCGCCGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGTCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.774

100

0.578
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.714

100

0.567
  pilB Vibrio cholerae strain A1552

51.146

100

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

99.117

0.5
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.385

100

0.496
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.065

94.523

0.369