Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ALSL_RS10665 Genome accession   NZ_AP018560
Coordinates   2267945..2269669 (+) Length   574 a.a.
NCBI ID   WP_126539009.1    Uniprot ID   A0A2Z6E6L4
Organism   Aerosticca soli strain Dysh456     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2262945..2274669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALSL_RS10655 (ALSL_2142) - 2263641..2265092 (-) 1452 WP_126539005.1 flagellin -
  ALSL_RS10660 (ALSL_2143) - 2266649..2267815 (-) 1167 WP_126539007.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  ALSL_RS10665 (ALSL_2144) pilB 2267945..2269669 (+) 1725 WP_126539009.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ALSL_RS10670 (ALSL_2147) - 2270759..2271205 (-) 447 WP_126539011.1 RES family NAD+ phosphorylase -
  ALSL_RS10675 (ALSL_2148) - 2271205..2271696 (-) 492 WP_161970950.1 antitoxin Xre/MbcA/ParS toxin-binding domain-containing protein -
  ALSL_RS10685 (ALSL_2149) - 2272059..2272988 (-) 930 WP_170172047.1 Nudix family hydrolase -
  ALSL_RS10690 (ALSL_2150) - 2273005..2273493 (-) 489 WP_126539015.1 dihydrofolate reductase -
  ALSL_RS10695 (ALSL_2151) - 2273543..2274337 (-) 795 WP_126539018.1 thymidylate synthase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62685.39 Da        Isoelectric Point: 6.2653

>NTDB_id=70067 ALSL_RS10665 WP_126539009.1 2267945..2269669(+) (pilB) [Aerosticca soli strain Dysh456]
MSSQPLSSLAGLSGMARRLVAEGVLPEPDVRRAVAESAQQKTSLLGWLFDQRLTDNLRLLQVAALEFGIPMLDIGTLSPA
SMPLDAISEELIARHQVLPLYRRGKRLYVAIADPMQSQALDEIKFHANCLVEPVLVERAALLRAIDNALANLHAAVPDMG
SDALNELALEAGDEEAEPTGIDATANDDAPVVKFVNKILVDAIKRGASDIHFEPFETQYRVRLRLDGILRPVASPPMKLA
NRIASRLKVMSGLDIAERRVPQDGRIKLNLSKSRAVDFRVSTLPTLFGEKIVLRILDGSTARLGIDALGYEDDQKQLYLE
AIHKPYGMVLVTGPTGSGKTVSLYTALNILNTPERNISTVEDPVEIRVEGINQVQQNTKRGMTFAAALRSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCTHCKREIELPEHAL
LAAGFTREEIAAGLTVYEAVGCDRCNDGYKGRVGIYQVMPMLEEIQQIVLQGGNALQIAEVARKAGVNDLRASALLKVKQ
GVTSLAEIDRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=70067 ALSL_RS10665 WP_126539009.1 2267945..2269669(+) (pilB) [Aerosticca soli strain Dysh456]
ATGTCGTCGCAACCGCTTTCCTCCCTTGCCGGGCTTTCCGGCATGGCACGCCGGCTGGTGGCCGAGGGCGTGCTGCCCGA
ACCGGACGTGCGCCGGGCGGTGGCCGAATCGGCGCAGCAGAAGACTTCGCTGCTGGGCTGGCTGTTCGACCAGCGCCTGA
CGGACAACCTGCGGTTGCTGCAGGTCGCCGCGCTGGAGTTTGGCATCCCCATGCTGGACATCGGTACGCTCTCGCCGGCG
TCCATGCCGCTCGATGCGATCAGCGAGGAGCTGATCGCCCGACATCAGGTGTTGCCGCTCTACCGCCGCGGCAAGCGGCT
GTACGTGGCCATCGCCGACCCGATGCAGTCGCAGGCGCTGGACGAGATCAAGTTCCACGCCAACTGCCTGGTGGAACCGG
TGCTGGTCGAGCGCGCGGCATTGCTGCGCGCGATCGACAACGCGCTGGCCAACCTGCACGCCGCGGTGCCGGACATGGGC
AGCGACGCGCTCAACGAACTGGCGCTCGAGGCCGGCGACGAGGAGGCCGAACCCACCGGCATCGACGCCACCGCCAACGA
CGACGCGCCGGTGGTGAAGTTCGTCAACAAGATCCTGGTCGATGCGATCAAGCGCGGCGCCTCGGACATCCATTTCGAAC
CGTTCGAGACGCAATACCGCGTGCGGCTGCGCCTGGACGGCATCCTGCGCCCGGTGGCCAGCCCGCCGATGAAACTGGCC
AACCGCATCGCCTCGCGCCTGAAGGTGATGAGCGGCCTGGACATCGCCGAGCGGCGCGTGCCGCAGGACGGCCGCATCAA
GCTCAACCTGTCCAAGAGCCGCGCGGTGGACTTTCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGATCGTGCTGC
GCATTCTGGACGGCTCCACGGCCAGACTCGGCATCGACGCGCTGGGCTACGAGGACGACCAGAAGCAGTTGTATCTCGAG
GCCATCCACAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTATACGGCGCT
CAACATCCTGAACACCCCGGAGCGCAACATCTCCACGGTGGAGGACCCGGTCGAGATCCGCGTGGAAGGCATCAACCAGG
TGCAGCAGAACACCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATG
GTCGGCGAGATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACTGGCCACATGGTGCTGTCCACGCT
GCACACCAACGACGCGCCGCAGACCATCGCCCGCCTGATGAACATGGGCATCGCCCCGTACAACATCACCAGTTCGGTGA
CGCTGGTGATCGCCCAGCGCCTGGCGCGGCGGCTGTGCACGCACTGCAAGCGCGAGATCGAACTGCCCGAGCACGCCCTG
CTCGCCGCCGGCTTCACCCGAGAGGAAATCGCCGCGGGCCTGACCGTGTACGAGGCGGTCGGCTGCGATCGCTGCAACGA
CGGCTACAAGGGCCGCGTGGGCATCTATCAGGTGATGCCGATGCTGGAGGAGATCCAGCAGATCGTGCTGCAGGGCGGCA
ACGCGCTGCAGATCGCCGAGGTGGCACGCAAGGCCGGCGTCAACGACCTGCGCGCCTCGGCGCTGCTCAAGGTCAAGCAG
GGCGTGACCAGCCTGGCCGAGATCGACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6E6L4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.752

98.432

0.549
  pilB Acinetobacter baumannii D1279779

54.833

99.129

0.544
  pilB Legionella pneumophila strain ERS1305867

52.622

99.652

0.524
  pilF Neisseria gonorrhoeae MS11

50.709

98.258

0.498
  pilB Vibrio cholerae strain A1552

51.504

92.683

0.477
  pilB Vibrio campbellii strain DS40M4

49.624

92.683

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

50.881

89.024

0.453
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.818

95.819

0.382
  pilF Thermus thermophilus HB27

41.075

90.767

0.373

Multiple sequence alignment