Detailed information NTG0700540

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: PCP29_RS06700
• Genome accession: NZ_CP115269
• Coordinates: 1409934..1410998 (+)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain F020
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1404934..1415998

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP29_RS06690 (PCP29_06685)	-	1405905..1407173 (+)	1269	WP_019726989.1	malic enzyme	-
PCP29_RS06695 (PCP29_06690)	-	1407278..1409749 (-)	2472	WP_019726988.1	penicillin-binding protein 1A	-
PCP29_RS06700 (PCP29_06695)	pilM	1409934..1410998 (+)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
PCP29_RS06705 (PCP29_06700)	pilN	1410998..1411594 (+)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
PCP29_RS06710 (PCP29_06705)	pilO	1411591..1412214 (+)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
PCP29_RS06715 (PCP29_06710)	comL/pilP	1412211..1412735 (+)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
PCP29_RS06720 (PCP29_06715)	pilQ	1412789..1414933 (+)	2145	WP_019726987.1	type 4a pilus secretin PilQ	Machinery gene
PCP29_RS06725 (PCP29_06720)	aroK	1414945..1415463 (+)	519	WP_003095833.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=700540 PCP29_RS06700 WP_003110888.1 1409934..1410998(+) (pilM) [Pseudomonas aeruginosa strain F020]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=700540 PCP29_RS06700 WP_003110888.1 1409934..1410998(+) (pilM) [Pseudomonas aeruginosa strain F020]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466