Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   NG743_RS04525 Genome accession   NZ_CP099464
Coordinates   999840..1000211 (-) Length   123 a.a.
NCBI ID   WP_027404669.1    Uniprot ID   A0A844ID78
Organism   Dolichospermum heterosporum TAC447 strain NIES-1697     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 994840..1005211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG743_RS04515 (NG743_04495) - 996370..999267 (-) 2898 WP_027404671.1 methyl-accepting chemotaxis protein -
  NG743_RS04520 (NG743_04500) - 999299..999832 (-) 534 WP_027404670.1 chemotaxis protein CheW -
  NG743_RS04525 (NG743_04505) pilH 999840..1000211 (-) 372 WP_027404669.1 response regulator transcription factor Machinery gene
  NG743_RS04530 (NG743_04510) - 1000390..1001613 (-) 1224 WP_027404668.1 response regulator -
  NG743_RS04535 (NG743_04515) hmpF 1002516..1004270 (+) 1755 WP_027404667.1 pilus motility taxis protein HmpF -

Sequence

Protein


Download         Length: 123 a.a.        Molecular weight: 13552.69 Da        Isoelectric Point: 5.1746

>NTDB_id=699823 NG743_RS04525 WP_027404669.1 999840..1000211(-) (pilH) [Dolichospermum heterosporum TAC447 strain NIES-1697]
MSTILIVEDSIAQREMITDLLKASGLKVTHASDGAEALEAIQTTPPDLVVLDIVMPRMNGYELCRRLKSDPKTQNVPVVM
CSSKGEEFDRYWGMKQGADAYIAKPFQPTELVGTVKQLLRGQG

Nucleotide


Download         Length: 372 bp        

>NTDB_id=699823 NG743_RS04525 WP_027404669.1 999840..1000211(-) (pilH) [Dolichospermum heterosporum TAC447 strain NIES-1697]
ATGAGTACAATTCTCATTGTTGAAGACAGTATTGCCCAGAGGGAGATGATTACAGACCTACTGAAAGCTAGTGGCTTAAA
AGTTACTCATGCCAGCGATGGTGCAGAAGCTTTAGAAGCAATTCAAACCACACCTCCCGATTTGGTGGTTTTGGATATTG
TCATGCCCCGCATGAACGGCTATGAACTCTGCCGACGATTAAAATCTGATCCTAAAACCCAAAATGTTCCTGTTGTCATG
TGTTCTTCTAAAGGTGAAGAATTTGATCGCTATTGGGGAATGAAACAAGGGGCAGATGCTTATATAGCAAAACCTTTCCA
ACCAACTGAATTGGTAGGAACAGTTAAACAACTCCTGCGAGGACAAGGATGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(4-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A844ID78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

75.207

98.374

0.74
  micA Streptococcus pneumoniae Cp1015

39.167

97.561

0.382
  vicR Streptococcus mutans UA159

38.333

97.561

0.374
  pilG Acinetobacter baumannii strain A118

39.474

92.683

0.366
  pilL-C Synechocystis sp. PCC 6803

37.19

98.374

0.366