Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   NFK05_RS27325 Genome accession   NZ_CP099306
Coordinates   5803563..5805083 (+) Length   506 a.a.
NCBI ID   WP_025108421.1    Uniprot ID   A0A0J2H2L0
Organism   Klebsiella michiganensis strain RHB20-SO-C01     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 5798563..5810083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NFK05_RS27305 (NFK05_27280) - 5800128..5801057 (-) 930 WP_004097473.1 branched-chain amino acid transaminase -
  NFK05_RS27310 (NFK05_27285) ilvM 5801074..5801331 (-) 258 WP_004097471.1 acetolactate synthase 2 small subunit -
  NFK05_RS27315 (NFK05_27290) ilvG 5801328..5802974 (-) 1647 WP_032747330.1 acetolactate synthase 2 catalytic subunit -
  NFK05_RS27320 (NFK05_27295) ilvL 5803114..5803212 (-) 99 WP_001311244.1 ilv operon leader peptide -
  NFK05_RS27325 (NFK05_27300) comM 5803563..5805083 (+) 1521 WP_025108421.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  NFK05_RS27330 (NFK05_27305) - 5805107..5805445 (-) 339 WP_004097464.1 DUF413 domain-containing protein -
  NFK05_RS27335 (NFK05_27310) hdfR 5805564..5806385 (+) 822 WP_004097454.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55211.68 Da        Isoelectric Point: 8.3570

>NTDB_id=698583 NFK05_RS27325 WP_025108421.1 5803563..5805083(+) (comM) [Klebsiella michiganensis strain RHB20-SO-C01]
MSLAIVYTRAALGIGAPLITVEVHISNGLPGLTMVGLPETTVREARDRVRSAIINSGYAYPAKKITINLAPADLPKEGGR
YDLPIALALLVASEQLKAPRLNQYEFVGELALTGALRGVPGAISSAMEAIKAGRKIVISNENSPEVGLIGGSDCLIATDL
QEVCAFLEGRHTLANPLPEGEYSDETSVDLRDVIGQRQGKRALEIIAAGGHNLLMIGPPGTGKTMLASRLPGLLPPLSNQ
EALESAAILSLVDSRHAMKNWRRRPFRAPHHSASLAAMVGGGSIPAPGEISLAHNGVLFLDELPEFERRVLDALREPIES
GVIHISRTRAKIDYPARFQLIAAMNPSPTGHYQGQHNRASPEQTLRYLGRLSGPFLDRFDLSLEIPLPPPGVLSQGTNEE
ESSACVRQRVLAARERQLSRQKKLNAHLGNDEMKICCRLHKDDAVWLEQTLIQLGLSIRAWQRLIKVSRTIADIEAVERI
ERHHLQEALGYRAIDRMLSHLQKMMA

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=698583 NFK05_RS27325 WP_025108421.1 5803563..5805083(+) (comM) [Klebsiella michiganensis strain RHB20-SO-C01]
ATGTCACTCGCCATTGTTTACACCCGCGCCGCTCTCGGGATCGGTGCCCCGCTTATCACGGTTGAAGTTCATATCAGCAA
CGGGCTCCCAGGTCTGACAATGGTAGGACTGCCTGAAACAACGGTTAGAGAAGCGCGTGACCGGGTACGTAGTGCGATTA
TCAACAGCGGTTACGCTTATCCGGCGAAAAAAATCACCATCAATCTGGCCCCGGCCGATCTACCAAAAGAAGGGGGAAGA
TATGATTTACCTATCGCTCTCGCGCTTCTGGTGGCCTCAGAGCAGCTAAAGGCCCCCAGGCTAAATCAATATGAGTTTGT
CGGCGAGCTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCTGGGGCCATATCCAGCGCCATGGAGGCCATAAAAGCGGGGC
GGAAGATTGTTATCTCTAATGAGAATTCTCCAGAGGTGGGGTTAATCGGCGGCAGTGACTGCCTTATCGCCACCGATCTC
CAGGAAGTCTGCGCTTTTCTCGAAGGTCGACATACCCTGGCTAACCCCCTTCCAGAAGGGGAATATTCGGATGAAACATC
GGTTGATCTGCGTGATGTTATTGGCCAGCGGCAGGGGAAACGAGCGCTGGAAATCATCGCTGCAGGTGGCCACAACCTGC
TGATGATAGGCCCGCCCGGCACAGGAAAAACGATGCTCGCCAGTCGATTACCGGGACTACTGCCGCCGCTGAGTAATCAG
GAAGCGCTGGAAAGCGCGGCAATACTTAGCCTGGTCGATTCCAGGCATGCCATGAAAAATTGGCGCCGCAGGCCCTTTCG
TGCCCCGCATCATAGCGCCTCCCTGGCGGCCATGGTGGGCGGAGGCTCAATTCCGGCACCGGGAGAGATCTCCCTTGCCC
ATAACGGAGTCCTGTTCCTGGATGAGCTGCCTGAATTCGAACGCAGGGTGCTGGATGCGTTACGCGAACCCATTGAGTCA
GGGGTTATCCATATCTCACGCACGCGCGCCAAAATCGATTATCCAGCCCGTTTCCAGCTTATTGCAGCGATGAATCCCAG
CCCGACAGGACACTATCAGGGCCAGCATAATCGCGCTTCACCGGAACAGACGCTACGCTACCTGGGTCGTTTATCCGGCC
CGTTTCTTGACCGATTCGATCTCTCCCTGGAGATCCCGCTTCCGCCTCCGGGGGTGCTCAGCCAGGGAACAAACGAAGAG
GAAAGTAGCGCCTGCGTACGCCAACGTGTTCTGGCCGCACGCGAAAGGCAGCTATCGCGGCAGAAAAAACTTAACGCGCA
TCTGGGAAATGATGAGATGAAAATCTGCTGCCGGCTTCATAAAGATGACGCTGTCTGGCTGGAGCAGACGCTAATACAGC
TCGGGCTTTCAATCCGCGCCTGGCAGCGCCTGATTAAAGTGTCCCGAACTATTGCCGATATTGAGGCAGTCGAACGCATC
GAACGTCATCATTTGCAGGAGGCTCTCGGCTATCGGGCAATAGACAGAATGTTGAGCCATCTACAAAAAATGATGGCGTA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J2H2L0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

59.1

100

0.597
  comM Vibrio cholerae strain A1552

59.841

99.407

0.595
  comM Glaesserella parasuis strain SC1401

58.974

100

0.591
  comM Vibrio campbellii strain DS40M4

59.363

99.209

0.589
  comM Legionella pneumophila str. Paris

48.185

98.024

0.472
  comM Legionella pneumophila strain ERS1305867

48.185

98.024

0.472
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

43.495

100

0.443