Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PCP27_RS07715 Genome accession   NZ_CP115243
Coordinates   1637184..1637723 (-) Length   179 a.a.
NCBI ID   WP_003116248.1    Uniprot ID   Q59651
Organism   Pseudomonas aeruginosa strain F043     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1632184..1642723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP27_RS07695 (PCP27_07685) - 1632868..1635123 (-) 2256 WP_033981335.1 DUF1631 domain-containing protein -
  PCP27_RS07700 (PCP27_07690) nadC 1635409..1636257 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP27_RS07710 (PCP27_07700) - 1636422..1637120 (-) 699 WP_023122772.1 hypothetical protein -
  PCP27_RS07715 (PCP27_07705) pilE 1637184..1637723 (-) 540 WP_003116248.1 pilin Machinery gene
  PCP27_RS07720 (PCP27_07710) pilB 1637952..1639655 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP27_RS07725 (PCP27_07715) pilC 1639659..1640876 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  PCP27_RS07730 (PCP27_07720) pilD 1640879..1641751 (+) 873 WP_016562340.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP27_RS07735 (PCP27_07725) coaE 1641748..1642359 (+) 612 WP_033982615.1 dephospho-CoA kinase -
  PCP27_RS07740 (PCP27_07730) yacG 1642356..1642556 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18003.27 Da        Isoelectric Point: 4.6894

>NTDB_id=698180 PCP27_RS07715 WP_003116248.1 1637184..1637723(-) (pilE) [Pseudomonas aeruginosa strain F043]
MTAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVQIAEATGEITVTFNAANVGNIPANSTLVFTPYVQNAAGAPTQLGASYASGVTGSIDWGCASDSNAVSSGTDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=698180 PCP27_RS07715 WP_003116248.1 1637184..1637723(-) (pilE) [Pseudomonas aeruginosa strain F043]
ATGACAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGCTTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACAAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCGCAAACGTGGGTAA
TATTCCGGCTAACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTTCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGACTCCAACGCGGTTTCCAGTGGTACCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCTAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q59651

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.451

100

0.447
  pilA Ralstonia pseudosolanacearum GMI1000

44.444

100

0.447
  pilA2 Legionella pneumophila str. Paris

42.373

98.883

0.419
  pilA2 Legionella pneumophila strain ERS1305867

41.808

98.883

0.413
  comP Acinetobacter baylyi ADP1

38.889

100

0.391