Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   UNDKW_RS04145 Genome accession   NZ_AP018439
Coordinates   919543..921270 (-) Length   575 a.a.
NCBI ID   WP_162057698.1    Uniprot ID   -
Organism   Undibacterium sp. KW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 914543..926270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UNDKW_RS04120 (UNDKW_0829) - 915685..915864 (-) 180 WP_162039882.1 DNA gyrase inhibitor YacG -
  UNDKW_RS04125 (UNDKW_0830) zapD 915867..916622 (-) 756 WP_162039883.1 cell division protein ZapD -
  UNDKW_RS04130 coaE 916797..917423 (-) 627 WP_162057695.1 dephospho-CoA kinase -
  UNDKW_RS04135 (UNDKW_0831) pilD 917432..918256 (-) 825 WP_370529082.1 A24 family peptidase Machinery gene
  UNDKW_RS04140 (UNDKW_0832) pilC 918292..919527 (-) 1236 WP_162057697.1 type II secretion system F family protein Machinery gene
  UNDKW_RS04145 (UNDKW_0833) pilF 919543..921270 (-) 1728 WP_162057698.1 type IV-A pilus assembly ATPase PilB Machinery gene
  UNDKW_RS04150 (UNDKW_0834) ispB 921595..922524 (-) 930 WP_162061753.1 octaprenyl diphosphate synthase -
  UNDKW_RS04155 (UNDKW_0835) rplU 922839..923150 (+) 312 WP_162020347.1 50S ribosomal protein L21 -
  UNDKW_RS04160 (UNDKW_0836) rpmA 923200..923469 (+) 270 WP_162039887.1 50S ribosomal protein L27 -
  UNDKW_RS04165 (UNDKW_0837) obgE 923723..924835 (+) 1113 WP_162057699.1 GTPase ObgE -
  UNDKW_RS04170 (UNDKW_0838) proB 925141..926259 (+) 1119 WP_162039889.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62295.67 Da        Isoelectric Point: 5.0318

>NTDB_id=69604 UNDKW_RS04145 WP_162057698.1 919543..921270(-) (pilF) [Undibacterium sp. KW1]
MSAVLPNSASQMPSSGLARALTQANLLTAAQVETVSKKAQTDKTGFIDALTQSGTVSARDLATFCSDAFGYPLLDLSLIN
ESLIPEKLVDQKLMQSQRLIAIAKKGNKISLAISDPTNNSALDQVKFQTGLGIDIIIVQHDALVKLLDKLGKSSEQSLNE
IGGDEFDLEFKEEDLGAAVAEAQTAEIDDAPVVKFLQKMLVDAINLGASDLHFEPFEKFYRIRFRVDGELREIAQPPLAI
KDKLSSRIKVISKLDISEKRIPQDGRMKLQLSASKSIDFRVSTLPTLFGEKIVMRILDGSQAQMGIDALGYDPDHKAILL
DAIQRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFAAALKSFLRQDPDVI
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPATLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKKPVDILDEVL
LAAGFKDDDLDGTWKPYGPVGCERCNGGGYKGRVGIYQLMPISESIERLILASASALDIEEQAQREGIRNLRQSGLMKVR
QGLTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=69604 UNDKW_RS04145 WP_162057698.1 919543..921270(-) (pilF) [Undibacterium sp. KW1]
ATGTCCGCAGTCTTACCAAATTCAGCATCCCAGATGCCAAGCTCTGGCTTGGCAAGAGCGTTGACGCAGGCCAATTTATT
GACTGCAGCCCAGGTTGAAACTGTCAGTAAAAAAGCGCAGACAGACAAAACCGGTTTTATTGATGCCCTGACGCAGAGCG
GCACGGTTAGCGCGCGCGACCTTGCCACCTTCTGTTCGGATGCCTTTGGCTATCCCTTGCTGGATTTATCACTGATCAAT
GAGTCCCTGATCCCGGAAAAACTGGTCGATCAAAAACTCATGCAATCACAGCGCCTGATTGCTATTGCCAAAAAAGGCAA
TAAAATATCCTTGGCGATCTCTGACCCAACCAACAACTCCGCCCTCGATCAGGTCAAGTTCCAGACCGGTCTGGGCATAG
ACATTATCATCGTGCAGCACGATGCTCTGGTTAAATTGCTGGATAAGCTGGGTAAATCTTCCGAGCAAAGCCTGAATGAA
ATTGGAGGAGATGAGTTTGATCTGGAATTCAAGGAAGAGGATCTGGGTGCAGCTGTTGCCGAGGCGCAGACCGCTGAAAT
TGATGATGCACCGGTCGTAAAATTCCTGCAAAAAATGCTGGTTGATGCCATTAATCTTGGCGCATCAGACTTGCATTTCG
AACCCTTTGAAAAATTTTACCGCATACGCTTCCGGGTGGATGGCGAGCTCCGTGAAATAGCGCAGCCGCCACTGGCCATC
AAGGACAAACTGTCTTCCCGTATCAAGGTTATCTCCAAACTCGACATTTCGGAAAAACGTATTCCGCAAGATGGCCGGAT
GAAATTGCAGTTATCTGCCAGCAAATCCATCGACTTTCGTGTCAGTACTCTGCCCACCCTGTTCGGTGAAAAGATCGTTA
TGCGTATTCTGGACGGCTCCCAGGCGCAGATGGGTATTGATGCTCTAGGCTACGACCCCGACCACAAAGCTATCCTGCTG
GATGCGATTCAACGCCCCTACGGCATGGTGCTGGTGACAGGACCTACAGGTTCGGGTAAAACCGTTTCTCTGTACACCTG
TCTCAATGTTATTAATAAACCTGGCATTAATATCTCTACTGCCGAAGATCCGGCGGAGATCAACTTGCCGGGGGTTAACC
AGGTTAACGTCAATGACAAGGCGGGCCTGACCTTTGCGGCTGCCCTGAAATCATTCTTGCGTCAGGATCCCGACGTCATC
ATGGTGGGTGAGATTCGCGACCTGGAAACTGCCGATATTGCGATCAAGGCTGCGCAAACTGGTCACATGGTATTTTCTAC
CTTGCATACGAATGATGCACCGGCCACCCTGACCCGTCTGATGAACATGGGTGTGGCACCGTTCAATATTGCTTCCTCTG
TCATTCTGATTACGGCGCAAAGGCTGGCACGCCGTTTATGCACCTGTAAAAAGCCTGTTGATATTCTGGATGAAGTCTTG
CTGGCAGCCGGCTTCAAGGATGATGATCTAGATGGCACATGGAAACCGTATGGCCCGGTAGGCTGTGAACGTTGCAACGG
CGGCGGTTACAAAGGTCGTGTCGGTATCTACCAGTTAATGCCTATCTCGGAATCGATAGAACGCCTGATTCTGGCCAGCG
CCAGCGCTCTTGATATTGAAGAACAGGCGCAGCGCGAAGGCATCAGGAATTTGCGACAATCCGGATTAATGAAAGTCAGA
CAGGGACTGACCAGCCTCGAGGAAGTCCTCGGATGTACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.805

98.261

0.529
  pilB Acinetobacter baumannii D1279779

53.819

97.913

0.527
  pilB Acinetobacter baylyi ADP1

52.575

97.913

0.515
  pilB Legionella pneumophila strain ERS1305867

50

98.087

0.49
  pilB Vibrio cholerae strain A1552

48.313

97.913

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

46.06

99.304

0.457
  pilB Vibrio campbellii strain DS40M4

45.374

97.739

0.443
  pilF Thermus thermophilus HB27

39.965

100

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.646

98.261

0.39

Multiple sequence alignment