Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9417_RS06730 Genome accession   NZ_CP097793
Coordinates   1353628..1355013 (+) Length   461 a.a.
NCBI ID   WP_014391364.1    Uniprot ID   -
Organism   Pasteurella multocida strain 21275     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1348628..1360013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9417_RS06700 (M9417_06700) - 1348819..1349670 (-) 852 WP_014391366.1 thymidylate synthase -
  M9417_RS06705 (M9417_06705) lgt 1349667..1350479 (-) 813 WP_014391365.1 prolipoprotein diacylglyceryl transferase -
  M9417_RS06710 (M9417_06710) - 1350489..1351283 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M9417_RS06715 (M9417_06715) rppH 1351286..1351882 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M9417_RS06720 (M9417_06720) ampD 1352468..1353034 (-) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9417_RS06725 (M9417_06725) pilA 1353194..1353628 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M9417_RS06730 (M9417_06730) pilB 1353628..1355013 (+) 1386 WP_014391364.1 GspE/PulE family protein Machinery gene
  M9417_RS06735 (M9417_06735) - 1355013..1356224 (+) 1212 WP_079157761.1 type II secretion system F family protein -
  M9417_RS06740 (M9417_06740) - 1356224..1356946 (+) 723 WP_041423102.1 A24 family peptidase -
  M9417_RS06745 (M9417_06745) coaE 1356961..1357581 (+) 621 WP_014391361.1 dephospho-CoA kinase -
  M9417_RS06750 (M9417_06750) yacG 1357592..1357795 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M9417_RS06755 (M9417_06755) - 1357795..1358064 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M9417_RS06760 (M9417_06760) - 1358513..1359886 (+) 1374 WP_005722571.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52255.91 Da        Isoelectric Point: 6.5608

>NTDB_id=692561 M9417_RS06730 WP_014391364.1 1353628..1355013(+) (pilB) [Pasteurella multocida strain 21275]
MFHENPLCTVSTMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPHFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFTELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=692561 M9417_RS06730 WP_014391364.1 1353628..1355013(+) (pilB) [Pasteurella multocida strain 21275]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTACAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAATATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCACTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTACTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCTCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGAAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.77

98.265

0.666
  pilB Haemophilus influenzae Rd KW20

67.329

98.265

0.662
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.04

100

0.406
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395