Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9412_RS08595 Genome accession   NZ_CP097789
Coordinates   1824860..1826248 (+) Length   462 a.a.
NCBI ID   WP_250262446.1    Uniprot ID   -
Organism   Pasteurella multocida strain 35564     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1819860..1831248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9412_RS08565 (M9412_08565) - 1820051..1820902 (-) 852 WP_005751072.1 thymidylate synthase -
  M9412_RS08570 (M9412_08570) lgt 1820908..1821711 (-) 804 WP_195188378.1 prolipoprotein diacylglyceryl transferase -
  M9412_RS08575 (M9412_08575) - 1821721..1822515 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M9412_RS08580 (M9412_08580) rppH 1822518..1823114 (-) 597 WP_250262444.1 RNA pyrophosphohydrolase -
  M9412_RS08585 (M9412_08585) ampD 1823700..1824266 (-) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9412_RS08590 (M9412_08590) pilA 1824426..1824860 (+) 435 WP_250262445.1 prepilin peptidase-dependent pilin Machinery gene
  M9412_RS08595 (M9412_08595) pilB 1824860..1826248 (+) 1389 WP_250262446.1 GspE/PulE family protein Machinery gene
  M9412_RS08600 (M9412_08600) pilC 1826248..1827459 (+) 1212 WP_250263136.1 type II secretion system F family protein Machinery gene
  M9412_RS08605 (M9412_08605) - 1827459..1828184 (+) 726 WP_250262447.1 A24 family peptidase -
  M9412_RS08610 (M9412_08610) coaE 1828196..1828807 (+) 612 WP_250262448.1 dephospho-CoA kinase -
  M9412_RS08615 (M9412_08615) yacG 1828827..1829030 (+) 204 WP_241951067.1 DNA gyrase inhibitor YacG -
  M9412_RS08620 (M9412_08620) - 1829030..1829299 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M9412_RS08625 (M9412_08625) - 1829748..1831121 (+) 1374 WP_250262449.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52121.63 Da        Isoelectric Point: 6.2350

>NTDB_id=692474 M9412_RS08595 WP_250262446.1 1824860..1826248(+) (pilB) [Pasteurella multocida strain 35564]
MFHENPLCTVSTMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEQQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPSKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRLQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSVEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCSDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=692474 M9412_RS08595 WP_250262446.1 1824860..1826248(+) (pilB) [Pasteurella multocida strain 35564]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCACAATGACAGGAGAGGTATTTGAAATCCAGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAACAACAGCTATGGCTTGGGG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACCAATAAGCACATTGAACCTGTTTTGTTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCATCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGATATTCATTTTGAACCGCAACAGCATAGTTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACGTTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCCGAGTTGGGGATG
ACCGCAGACCAACAACACAGGCTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACAGTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTCTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGTGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACTGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.55

98.052

0.662
  pilB Haemophilus influenzae Rd KW20

67.108

98.052

0.658
  pilB Glaesserella parasuis strain SC1401

58.59

98.268

0.576
  pilB Acinetobacter baumannii D1279779

39.554

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.917

100

0.416
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.411
  pilB Acinetobacter baylyi ADP1

37.675

100

0.407
  pilB Vibrio cholerae strain A1552

38.477

100

0.405
  pilB Legionella pneumophila strain ERS1305867

36.605

100

0.387