Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9421_RS08595 Genome accession   NZ_CP097788
Coordinates   1830366..1830800 (+) Length   144 a.a.
NCBI ID   WP_250262445.1    Uniprot ID   -
Organism   Pasteurella multocida strain 21317     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1825366..1835800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9421_RS08565 (M9421_08565) tadA 1825476..1825976 (-) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -
  M9421_RS08570 (M9421_08570) - 1825991..1826842 (-) 852 WP_005751072.1 thymidylate synthase -
  M9421_RS08575 (M9421_08575) lgt 1826848..1827651 (-) 804 WP_195188378.1 prolipoprotein diacylglyceryl transferase -
  M9421_RS08580 (M9421_08580) - 1827661..1828455 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M9421_RS08585 (M9421_08585) rppH 1828458..1829054 (-) 597 WP_250262444.1 RNA pyrophosphohydrolase -
  M9421_RS08590 (M9421_08590) ampD 1829640..1830206 (-) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9421_RS08595 (M9421_08595) pilA 1830366..1830800 (+) 435 WP_250262445.1 prepilin peptidase-dependent pilin Machinery gene
  M9421_RS08600 (M9421_08600) pilB 1830800..1832188 (+) 1389 WP_250262446.1 GspE/PulE family protein Machinery gene
  M9421_RS08605 (M9421_08605) pilC 1832188..1833399 (+) 1212 WP_250263136.1 type II secretion system F family protein Machinery gene
  M9421_RS08610 (M9421_08610) - 1833399..1834124 (+) 726 WP_250262447.1 A24 family peptidase -
  M9421_RS08615 (M9421_08615) coaE 1834136..1834747 (+) 612 WP_250262448.1 dephospho-CoA kinase -
  M9421_RS08620 (M9421_08620) yacG 1834767..1834970 (+) 204 WP_241951067.1 DNA gyrase inhibitor YacG -
  M9421_RS08625 (M9421_08625) - 1834970..1835239 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15044.45 Da        Isoelectric Point: 9.1281

>NTDB_id=692450 M9421_RS08595 WP_250262445.1 1830366..1830800(+) (pilA) [Pasteurella multocida strain 21317]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAAQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=692450 M9421_RS08595 WP_250262445.1 1830366..1830800(+) (pilA) [Pasteurella multocida strain 21317]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCTATTATTGCGAT
TTTAGCCACGATTGCTGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.889

100

0.639
  pilA Haemophilus influenzae Rd KW20

61.806

100

0.618
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

35.333

100

0.368