Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M9411_RS02000 Genome accession   NZ_CP097787
Coordinates   432143..433528 (-) Length   461 a.a.
NCBI ID   WP_250252228.1    Uniprot ID   -
Organism   Pasteurella multocida strain 38725     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 427143..438528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9411_RS01970 (M9411_01970) - 427270..428643 (-) 1374 WP_250252222.1 sodium:alanine symporter family protein -
  M9411_RS01975 (M9411_01975) - 429092..429361 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M9411_RS01980 (M9411_01980) yacG 429361..429564 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M9411_RS01985 (M9411_01985) coaE 429575..430195 (-) 621 WP_250252224.1 dephospho-CoA kinase -
  M9411_RS01990 (M9411_01990) - 430210..430932 (-) 723 WP_250252226.1 A24 family peptidase -
  M9411_RS01995 (M9411_01995) - 430932..432143 (-) 1212 WP_005722560.1 type II secretion system F family protein -
  M9411_RS02000 (M9411_02000) pilB 432143..433528 (-) 1386 WP_250252228.1 GspE/PulE family protein Machinery gene
  M9411_RS02005 (M9411_02005) pilA 433528..433962 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M9411_RS02010 (M9411_02010) ampD 434122..434688 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9411_RS02015 (M9411_02015) rppH 435274..435870 (+) 597 WP_250252230.1 RNA pyrophosphohydrolase -
  M9411_RS02020 (M9411_02020) - 435873..436667 (+) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  M9411_RS02025 (M9411_02025) lgt 436677..437480 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M9411_RS02030 (M9411_02030) - 437486..438337 (+) 852 WP_005751072.1 thymidylate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52158.89 Da        Isoelectric Point: 6.4990

>NTDB_id=692408 M9411_RS02000 WP_250252228.1 432143..433528(-) (pilB) [Pasteurella multocida strain 38725]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPPFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIVLTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVRRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=692408 M9411_RS02000 WP_250252228.1 432143..433528(-) (pilB) [Pasteurella multocida strain 38725]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGTGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGCG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTGAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTTCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTCCCTTTTCGGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACGCATTTAGGCGAAAAAGCGGTATTACGTTTGCAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAACACAGGTTCCAACACGCGTTAAGTCAACCTCAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGTGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCGGC
TCAAACAGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACGACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

68.212

98.265

0.67
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.59

98.482

0.577
  pilB Acinetobacter baumannii D1279779

40

100

0.43
  pilB Vibrio parahaemolyticus RIMD 2210633

39.545

100

0.414
  pilB Acinetobacter baylyi ADP1

38.124

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.375

100

0.41
  pilB Vibrio cholerae strain A1552

38.604

100

0.408
  pilB Legionella pneumophila strain ERS1305867

37.678

100

0.401