Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M8852_RS05770 Genome accession   NZ_CP097614
Coordinates   1244844..1245278 (-) Length   144 a.a.
NCBI ID   WP_115097851.1    Uniprot ID   -
Organism   Pasteurella multocida strain P1581     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1239844..1250278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8852_RS05740 (M8852_05740) - 1240405..1240674 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M8852_RS05745 (M8852_05745) yacG 1240674..1240877 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  M8852_RS05750 (M8852_05750) coaE 1240888..1241508 (-) 621 WP_115097849.1 dephospho-CoA kinase -
  M8852_RS05755 (M8852_05755) - 1241520..1242245 (-) 726 WP_096743228.1 A24 family peptidase -
  M8852_RS05760 (M8852_05760) pilC 1242245..1243456 (-) 1212 WP_096743229.1 type II secretion system F family protein Machinery gene
  M8852_RS05765 (M8852_05765) pilB 1243456..1244844 (-) 1389 WP_115097850.1 GspE/PulE family protein Machinery gene
  M8852_RS05770 (M8852_05770) pilA 1244844..1245278 (-) 435 WP_115097851.1 prepilin peptidase-dependent pilin Machinery gene
  M8852_RS05775 (M8852_05775) ampD 1245438..1246004 (+) 567 WP_104227618.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M8852_RS05780 (M8852_05780) rppH 1246590..1247186 (+) 597 WP_115097852.1 RNA pyrophosphohydrolase -
  M8852_RS05785 (M8852_05785) - 1247189..1247983 (+) 795 WP_115097853.1 sulfite exporter TauE/SafE family protein -
  M8852_RS05790 (M8852_05790) lgt 1247993..1248796 (+) 804 WP_115097854.1 prolipoprotein diacylglyceryl transferase -
  M8852_RS05795 (M8852_05795) - 1248802..1249653 (+) 852 WP_115097855.1 thymidylate synthase -
  M8852_RS05800 (M8852_05800) tadA 1249680..1250168 (+) 489 WP_083004788.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15072.51 Da        Isoelectric Point: 9.1281

>NTDB_id=691237 M8852_RS05770 WP_115097851.1 1244844..1245278(-) (pilA) [Pasteurella multocida strain P1581]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=691237 M8852_RS05770 WP_115097851.1 1244844..1245278(-) (pilA) [Pasteurella multocida strain P1581]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCTGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATTTCTGAATTATTGCAAGCCGCAGCTC
CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

35.333

100

0.368