Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M8331_RS06035 Genome accession   NZ_CP097604
Coordinates   1286986..1287420 (-) Length   144 a.a.
NCBI ID   WP_083004804.1    Uniprot ID   A0A1Q1PRS7
Organism   Pasteurella multocida strain 10159     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1281986..1292420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8331_RS06005 (M8331_06005) - 1282550..1282819 (-) 270 WP_250190736.1 GNAT family N-acetyltransferase -
  M8331_RS06010 (M8331_06010) yacG 1282819..1283022 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  M8331_RS06015 (M8331_06015) coaE 1283033..1283653 (-) 621 WP_083004815.1 dephospho-CoA kinase -
  M8331_RS06020 (M8331_06020) - 1283665..1284390 (-) 726 WP_083004812.1 A24 family peptidase -
  M8331_RS06025 (M8331_06025) pilC 1284390..1285601 (-) 1212 WP_083004809.1 type II secretion system F family protein Machinery gene
  M8331_RS06030 (M8331_06030) pilB 1285601..1286986 (-) 1386 WP_083004806.1 GspE/PulE family protein Machinery gene
  M8331_RS06035 (M8331_06035) pilA 1286986..1287420 (-) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  M8331_RS06040 (M8331_06040) ampD 1287580..1288146 (+) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M8331_RS06045 (M8331_06045) rppH 1288732..1289328 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  M8331_RS06050 (M8331_06050) - 1289331..1290125 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  M8331_RS06055 (M8331_06055) lgt 1290135..1290938 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  M8331_RS06060 (M8331_06060) - 1290944..1291795 (+) 852 WP_250190737.1 thymidylate synthase -
  M8331_RS06065 (M8331_06065) tadA 1291822..1292310 (+) 489 WP_083004788.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15072.46 Da        Isoelectric Point: 8.8040

>NTDB_id=691061 M8331_RS06035 WP_083004804.1 1286986..1287420(-) (pilA) [Pasteurella multocida strain 10159]
MKKAIFFSIQKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=691061 M8331_RS06035 WP_083004804.1 1286986..1287420(-) (pilA) [Pasteurella multocida strain 10159]
ATGAAAAAAGCCATTTTCTTTTCTATTCAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q1PRS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

34.437

100

0.361