Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LNQ82_RS08130 Genome accession   NZ_CP097501
Coordinates   1606487..1608166 (-) Length   559 a.a.
NCBI ID   WP_027021067.1    Uniprot ID   A0AAE9KZL2
Organism   Conchiformibius steedae DSM 2580     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1601487..1613166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LNQ82_RS08105 (LNQ82_08105) - 1601551..1602786 (+) 1236 WP_169733441.1 YihY family inner membrane protein -
  LNQ82_RS08110 (LNQ82_08110) zapD 1602831..1603580 (-) 750 WP_027021063.1 cell division protein ZapD -
  LNQ82_RS08115 (LNQ82_08115) coaE 1603605..1604210 (-) 606 WP_027021064.1 dephospho-CoA kinase -
  LNQ82_RS08120 (LNQ82_08120) pilD 1604214..1605125 (-) 912 WP_027021065.1 prepilin peptidase Machinery gene
  LNQ82_RS08125 (LNQ82_08125) pilG 1605130..1606398 (-) 1269 WP_027021066.1 type II secretion system F family protein Machinery gene
  LNQ82_RS08130 (LNQ82_08130) pilF 1606487..1608166 (-) 1680 WP_027021067.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LNQ82_RS08135 (LNQ82_08135) mtgA 1608294..1608980 (-) 687 WP_027021068.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LNQ82_RS08140 (LNQ82_08140) aroE 1609118..1609918 (-) 801 Protein_1564 shikimate dehydrogenase -
  LNQ82_RS08145 (LNQ82_08145) hisC 1610206..1611267 (+) 1062 WP_027021069.1 histidinol-phosphate transaminase -
  LNQ82_RS08150 (LNQ82_08150) - 1611255..1612079 (-) 825 WP_027021070.1 hypothetical protein -
  LNQ82_RS08155 (LNQ82_08155) hisB 1612179..1612769 (+) 591 Protein_1567 imidazoleglycerol-phosphate dehydratase HisB -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62241.67 Da        Isoelectric Point: 5.3064

>NTDB_id=689754 LNQ82_RS08130 WP_027021067.1 1606487..1608166(-) (pilF) [Conchiformibius steedae DSM 2580]
MSIGLFRVLLQQKLIPQEKVSKYQQAAENNSFLQTIFNDKIITPQDLSSLLSRMFNYPQFNLNAFSRRGLMTDLLDENQM
AEHRCVPLFKRGKKLFMGVSDPTNIQALQKLVFSSGLSVDLVLVRDDHLSNFLEWFGQSSTSLLREMGADSALQEAQAQT
MIVDGEEEEDGPIARFIQKVLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPLNLRSQLASRIKVMAKLDISER
RVPQDGRIQLQFGKGTKAIDFRVNTMPCLFGEKVVMRILNSDAATLNIDQLGFEDFQKELIMEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLARRLCSSCKEPMERPPEPALKRAGFTDEDLAGDWVM
YRPVGCEVCKGKGYKGRAGLYEVMPMTEKMKAVIMNNGTEVDIANIAYEEGLVDLRRSGLLKVMQGITSLEEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=689754 LNQ82_RS08130 WP_027021067.1 1606487..1608166(-) (pilF) [Conchiformibius steedae DSM 2580]
ATGAGCATCGGTTTATTTCGTGTTCTGCTGCAACAGAAGCTGATACCGCAAGAAAAAGTCAGCAAATACCAACAAGCAGC
CGAAAACAACAGCTTTCTGCAAACCATTTTTAACGACAAAATCATTACCCCCCAAGATTTGTCGTCTTTGCTGTCGCGCA
TGTTCAACTACCCGCAGTTTAACCTTAATGCGTTTTCGCGGCGCGGGCTGATGACCGATTTGCTGGACGAAAACCAAATG
GCGGAACACCGCTGCGTACCTTTGTTCAAACGCGGCAAAAAACTGTTTATGGGCGTATCCGACCCCACCAATATCCAAGC
ACTGCAAAAACTGGTGTTTTCCAGCGGTTTGTCTGTTGATTTGGTGCTGGTGCGCGATGACCACTTAAGCAATTTTCTGG
AATGGTTCGGACAAAGCAGCACCAGCCTGTTGCGCGAAATGGGCGCAGACAGCGCCTTGCAGGAAGCCCAGGCGCAAACC
ATGATTGTGGACGGCGAGGAAGAAGAAGACGGTCCCATCGCCCGCTTTATCCAAAAAGTCTTGTCAGACGCGCTCAATGC
AGGCGCATCGGATATCCATTTTGAATTTTACGAACAAATGGCACGGGTGCGTTTCCGTGTGGACGGTCAGTTGCGCGAAG
TGGTGCAACCGCCGCTCAACCTGCGTAGCCAGTTGGCATCGCGTATTAAAGTAATGGCAAAACTGGATATTTCCGAACGA
CGCGTACCGCAGGACGGACGTATCCAGCTGCAATTTGGTAAAGGCACGAAAGCCATTGACTTCCGTGTGAACACCATGCC
CTGCCTGTTTGGCGAAAAAGTGGTGATGCGTATTTTGAACTCCGATGCCGCCACGCTGAACATCGACCAACTGGGTTTTG
AAGACTTCCAAAAAGAATTGATTATGGAAGCCATTCACCGTCCTTACGGCATGGTGTTGGTAACAGGTCCTACGGGTTCG
GGTAAAACCGTATCACTTTATACCTGCCTGAACATTCTGAATACCGACGATGTGAACATTTCCACCGCCGAAGACCCCGC
CGAGATTAACTTACCTGGTATCAATCAGGTAAACGTCAACGACAAACAGGGCTTAACCTTTGAAGCGGCACTCAAAGCCT
TCCTGCGTCAAGACCCTGACATCATCATGATTGGTGAGATTCGTGACTTGGGTACTGCCGACATCGCCATTAAAGCCGCC
CAAACGGGTCACATGGTATTTTCCACCCTGCACACCAACAACGCCCCCGCCACCCTGTCGCGTATGCTGAACATGGGTGT
TGCCCCGTTTAACATCGCCAGTTCCGTCAATCTAATTATGGCGCAGCGTTTGGCACGTCGTCTCTGTTCTTCCTGCAAAG
AACCCATGGAACGTCCGCCCGAACCCGCCCTCAAACGCGCAGGCTTTACCGATGAAGATTTGGCAGGCGATTGGGTGATG
TATCGTCCTGTAGGCTGCGAAGTGTGCAAAGGCAAAGGCTATAAAGGACGGGCGGGTTTGTATGAAGTGATGCCGATGAC
CGAAAAAATGAAAGCCGTGATTATGAACAACGGTACCGAAGTCGATATTGCCAATATTGCCTACGAAGAAGGCTTGGTGG
ATTTGCGCCGTTCCGGTTTGCTGAAAGTGATGCAAGGCATTACCTCGCTTGAAGAAGTGATTGCCACCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-140)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.036

100

0.682
  pilB Acinetobacter baumannii D1279779

52.135

100

0.524
  pilB Acinetobacter baylyi ADP1

51.805

99.106

0.513
  pilB Legionella pneumophila strain ERS1305867

47.965

100

0.485
  pilB Vibrio cholerae strain A1552

45.745

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.458
  pilB Vibrio campbellii strain DS40M4

45.273

98.39

0.445