Detailed information NTG0068970

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: SPYKS030_RS00685
• Genome accession: NZ_AP018337
• Coordinates: 105116..105550 (+)
• Length: 144 a.a.
• NCBI ID: WP_032462142.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain KS030
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 100116..110550

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYKS030_RS00655 (SPYKS030_00800)	-	101785..102150 (+)	366	WP_002986560.1	DUF1033 family protein	-
SPYKS030_RS00660 (SPYKS030_00810)	comYA	102243..103181 (+)	939	WP_011054114.1	competence type IV pilus ATPase ComGA	Machinery gene
SPYKS030_RS00665 (SPYKS030_00820)	comYB	103117..104151 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
SPYKS030_RS00670 (SPYKS030_00830)	comYC	104153..104479 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
SPYKS030_RS00675 (SPYKS030_00840)	comGD	104454..104882 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
SPYKS030_RS00680 (SPYKS030_00850)	comGE	104839..105123 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
SPYKS030_RS00685 (SPYKS030_00860)	comYF	105116..105550 (+)	435	WP_032462142.1	competence type IV pilus minor pilin ComGF	Machinery gene
SPYKS030_RS00690 (SPYKS030_00870)	comGG	105534..105860 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
SPYKS030_RS00695 (SPYKS030_00880)	comYH	105958..106911 (+)	954	WP_011054117.1	class I SAM-dependent methyltransferase	Machinery gene
SPYKS030_RS00700 (SPYKS030_00890)	-	106970..108166 (+)	1197	WP_010921803.1	acetate kinase	-
SPYKS030_RS00705 (SPYKS030_00900)	-	108352..108660 (+)	309	WP_011054118.1	hypothetical protein	-
SPYKS030_RS00710 (SPYKS030_00910)	proC	108743..109513 (-)	771	WP_002992745.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16675.29 Da
• Isoelectric Point: 10.2526

>NTDB_id=68970 SPYKS030_RS00685 WP_032462142.1 105116..105550(+) (comYF) [Streptococcus pyogenes strain KS030]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYTFQD

Nucleotide

• Length: 435 bp

>NTDB_id=68970 SPYKS030_RS00685 WP_032462142.1 105116..105550(+) (comYF) [Streptococcus pyogenes strain KS030]
TTGAGTAAACAATTAAGTAATATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGCTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATACCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	50.36	96.528	0.486
comYF	Streptococcus mutans UA159	49.64	96.528	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396