Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   SPYKS030_RS00660 Genome accession   NZ_AP018337
Coordinates   102243..103181 (+) Length   312 a.a.
NCBI ID   WP_011054114.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain KS030     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97243..108181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYKS030_RS00650 (SPYKS030_00790) rpoC 98009..101632 (+) 3624 WP_011054112.1 DNA-directed RNA polymerase subunit beta' -
  SPYKS030_RS00655 (SPYKS030_00800) - 101785..102150 (+) 366 WP_002986560.1 DUF1033 family protein -
  SPYKS030_RS00660 (SPYKS030_00810) comYA 102243..103181 (+) 939 WP_011054114.1 competence type IV pilus ATPase ComGA Machinery gene
  SPYKS030_RS00665 (SPYKS030_00820) comYB 103117..104151 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  SPYKS030_RS00670 (SPYKS030_00830) comYC 104153..104479 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  SPYKS030_RS00675 (SPYKS030_00840) comGD 104454..104882 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  SPYKS030_RS00680 (SPYKS030_00850) comGE 104839..105123 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  SPYKS030_RS00685 (SPYKS030_00860) comYF 105116..105550 (+) 435 WP_032462142.1 competence type IV pilus minor pilin ComGF Machinery gene
  SPYKS030_RS00690 (SPYKS030_00870) comGG 105534..105860 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  SPYKS030_RS00695 (SPYKS030_00880) comYH 105958..106911 (+) 954 WP_011054117.1 class I SAM-dependent methyltransferase Machinery gene
  SPYKS030_RS00700 (SPYKS030_00890) - 106970..108166 (+) 1197 WP_010921803.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35076.28 Da        Isoelectric Point: 8.2452

>NTDB_id=68967 SPYKS030_RS00660 WP_011054114.1 102243..103181(+) (comYA) [Streptococcus pyogenes strain KS030]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRDSLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=68967 SPYKS030_RS00660 WP_011054114.1 102243..103181(+) (comYA) [Streptococcus pyogenes strain KS030]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAGTTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGATAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.415

99.679

0.612
  comYA Streptococcus mutans UA140

61.415

99.679

0.612
  comYA Streptococcus gordonii str. Challis substr. CH1

60.45

99.679

0.603
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.161

99.679

0.5
  comGA Latilactobacillus sakei subsp. sakei 23K

39.31

92.949

0.365

Multiple sequence alignment