Detailed information    

experimental Experimentally validated

Overview

Name   recD   Type   Machinery gene
Locus tag   NGFG_RS04110 Genome accession   NC_022240
Coordinates   767553..769298 (+) Length   581 a.a.
NCBI ID   WP_003688652.1    Uniprot ID   O68924
Organism   Neisseria gonorrhoeae MS11     
Function   homologous recombination   
Homologous recombination

Function

Mutations in the Gc recB, recC or recD genes revealed that a Gc RecBCD pathway also exists and is involved in DNA transformation and DNA repair but not in pilin antigenic variation.


Genomic Context

Location: 762553..774298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS04085 (NGFG_01024) - 762769..764307 (+) 1539 WP_003695299.1 SurA N-terminal domain-containing protein -
  NGFG_RS04090 (NGFG_01023) recR 764374..764991 (+) 618 WP_003688660.1 recombination mediator RecR Machinery gene
  NGFG_RS14335 (NGFG_01022) - 765047..765325 (-) 279 WP_003688659.1 hypothetical protein -
  NGFG_RS04100 (NGFG_01020) - 765551..766798 (+) 1248 WP_003691197.1 lipoprotein-releasing ABC transporter permease subunit -
  NGFG_RS04105 (NGFG_01019) lolD 766791..767486 (+) 696 WP_003688655.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  NGFG_RS04110 (NGFG_01018) recD 767553..769298 (+) 1746 WP_003688652.1 exodeoxyribonuclease V subunit alpha Machinery gene
  NGFG_RS04115 - 769370..770016 (-) 647 Protein_783 methyltransferase -
  NGFG_RS04120 (NGFG_02316) - 770024..770986 (+) 963 WP_025463956.1 IS110 family transposase -
  NGFG_RS15050 - 771115..771574 (-) 460 Protein_785 methyltransferase -
  NGFG_RS04130 (NGFG_01014) lon 771826..774288 (+) 2463 WP_003688644.1 endopeptidase La -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63039.83 Da        Isoelectric Point: 6.0083

>NTDB_id=1483 NGFG_RS04110 WP_003688652.1 767553..769298(+) (recD) [Neisseria gonorrhoeae MS11]
MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQNGHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRL
FLGRMWQLEYDLAAEIKRLAAAGTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
LICGENENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLLKLSPPKMQAAFDHIRPLPFDVL
IVDEASMLDTALMLQLLKAVKTGARVILLGDENQLPSVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLA
QNTAHLSFSHRFGDNSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGNIEAAYAGI
SDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYALELFNGDIGLIMEDVGRQGSLAAYFADADG
FKKVAVSCLPEFEPAFAMTVHKSQGSEYREVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAV
NTVKVRQTALGSMLERVFSQE

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=1483 NGFG_RS04110 WP_003688652.1 767553..769298(+) (recD) [Neisseria gonorrhoeae MS11]
ATGGAACTGCAGACAGATGAATTTGCCCAAGCGGCGGCAAGGGCGGCAATCCGTTTTTTGGAACGCTATGCGGGTTCGGG
CAACGAAGTGCTTGCAAACTGTACCGAACGCCTGTTTCAGGCATTGCAAAACGGTCATTCGTTTATCCGTTTGAGCGGGG
ACGAGGCTGACGCGCTGTCGGCACTCGCACCCGTGGTCGGAACATCCGCCGCGCCTTTGATTTTGGAAGGCAGAAGGCTG
TTTTTGGGCAGGATGTGGCAGTTGGAATACGATTTGGCTGCCGAAATAAAACGCTTGGCGGCAGCCGGCACGTCCGCCCC
CGACGCGGCAGGCGCGCGGCAAAACCTCGCAAAATGGTTTCAAGGCGCGGGAAGCGAAGGACAGCGCGATGCGGCCGCCT
TGGCACTGTTGCAGTTTTTTATGGTGATTACCGGAGGGCCGGGAACGGGCAAAACGACAACGGTTGCCAAACTGCTGGCA
CTGATTTGCGGTGAAAACGAAAATCTTCCCCATATCGCGCTTGCCGCACCGACGGGCAAAGCGGCGGCACATATGGCGCG
CGCGCTGCACCGTGCAATCAACGGTTTTGACGCGCCGGAGGCCGTCCGCCGCCATTTGCTCAAACTGGAAGGGCAAACCG
TCCACCGACTGCTTAAGCTGAGCCCGCCCAAAATGCAGGCGGCGTTCGACCATATCCGTCCGCTGCCGTTCGACGTGCTG
ATTGTCGATGAAGCCTCCATGTTGGATACCGCATTGATGCTGCAACTTTTGAAAGCGGTCAAAACCGGCGCGCGCGTGAT
TCTGCTGGGCGATGAAAACCAGCTCCCGTCCGTCGGAATCGGGGCGGTGCTGTCGGTTTTGTCGCAAAAAACCGTTTTGG
ACGGAGAAACGCACCAAAGGCTGGCCGGTTTCCTTCCGGAACACGGTTTCAGCGTCAGCGCAAATCCGCCCGTGTTGGCG
CAAAACACCGCCCATCTGTCGTTCAGCCACCGCTTCGGCGACAACAGCGGCATCGGCTGCCTTGCCCGTGCCGCCGTATC
GGGCGATGAAGGGGCGTGGGCATTGTTTGACCGGTTTCCGGACGAACTGGAACATTCGGAATGCAGTCCGAATGCTCGGG
TCGAAAGGTTGTACCGGGCGCACAAAGCCTATTGGCAGGCGGTAAAAGACGGCAATATCGAAGCCGCATACGCAGGCATC
TCGGATATCGTGGTTCTGGCAGCTTGGCGGCAGGATGCGGAAGACTTCAACGAAGCCTATTGCAGCTATGTACGCCGCAA
AATGAACATACCGGAACATTTGGCCTATTTCGCCGGAGAGCCGATTATGATCAGACAGAACGACTACGCGCTTGAGCTGT
TCAACGGCGACATCGGACTGATTATGGAAGATGTCGGACGGCAGGGCAGCCTTGCCGCCTATTTTGCCGATGCGGACGGA
TTTAAAAAGGTAGCGGTAAGCTGCTTGCCCGAATTTGAACCCGCATTCGCCATGACCGTCCACAAAAGCCAAGGTTCGGA
ATATCGGGAAGTCTGGCTGCTGCCGCCTTCCGACGCACCTTCGGACGAAGGGGACGATGCATTGTCCGGATTGAGTAAGG
AGCTGTTATATACCGCCATTACCCGCGCCAGGGAAAAATTCGTATTTTTCGGCGGCAAAAAGACATTTTGTCAGGCGGTA
AACACCGTCAAAGTCCGTCAGACGGCGTTGGGCAGTATGCTTGAGCGGGTATTTTCACAAGAATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_30)

(125-290)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(494-548)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O68924

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recD Neisseria gonorrhoeae strain FA1090

99.828

100

0.998

References

[1] I J Mehr et al. (1998) Differential roles of homologous recombination pathways in Neisseria gonorrhoeae pilin antigenic variation, DNA transformation and DNA repair. Molecular Microbiology 30(4):697-710. [PMID: 10094619]