Detailed information NTG0001134

Experimentally validated

Overview

• Name: recD
• Type: Machinery gene
• Locus tag: OK783_RS03985
• Genome accession: NZ_CP115654
• Coordinates: 765028..766773 (+)
• Length: 581 a.a.
• NCBI ID: WP_010358120.1
• Uniprot ID: Q5F8K1
• Organism: Neisseria gonorrhoeae strain FA1090
• Function: homologous recombination Homologous recombination

Function

Mutations in the Gc recB, recC or recD genes revealed that a Gc RecBCD pathway also exists and is involved in DNA transformation and DNA repair but not in pilin antigenic variation.

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	767460..768461	765028..766773	flank	687

Gene organization within MGE regions

Location: 765028..768461

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OK783_RS03985 (OK783_03985)	recD	765028..766773 (+)	1746	WP_010358120.1	exodeoxyribonuclease V subunit alpha	Machinery gene
OK783_RS03990 (OK783_03990)	-	766845..767491 (-)	647	Protein_775	methyltransferase	-
OK783_RS03995 (OK783_03995)	-	767499..768461 (+)	963	WP_157147402.1	IS110 family transposase	-

Sequence

Protein

• Length: 581 a.a.
• Molecular weight: 63055.83 Da
• Isoelectric Point: 6.0083

>NTDB_id=1134 OK783_RS03985 WP_010358120.1 765028..766773(+) (recD) [Neisseria gonorrhoeae strain FA1090]
MELQTDEFAQAAARAAIRFLERYAGSGNEVLANCTERLFQALQNGHSFIRLSGDEADALSALAPVVGTSAAPLILEGRRL
FLGRMWQLEYDLAAEIKRLAAAGTSAPDAAGARQNLAKWFQGAGSEGQRDAAALALLQFFMVITGGPGTGKTTTVAKLLA
LICGENENLPHIALAAPTGKAAAHMARALHRSINGFDAPEAVRRHLLKLEGQTVHRLLKLSPPKMQAAFDHIRPLPFDVL
IVDEASMLDTALMLQLLKAVKTGARVILLGDENQLPSVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLA
QNTAHLSFSHRFGDNSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGNIEAAYAGI
SDIVVLAAWRQDAEDFNEAYCSYVRRKMNIPEHLAYFAGEPIMIRQNDYALELFNGDIGLIMEDVGRQGSLAAYFADADG
FKKVAVSCLPEFEPAFAMTVHKSQGSEYREVWLLPPSDAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGKKTFCQAV
NTVKVRQTALGSMLERVFSQE

Nucleotide

• Length: 1746 bp

>NTDB_id=1134 OK783_RS03985 WP_010358120.1 765028..766773(+) (recD) [Neisseria gonorrhoeae strain FA1090]
ATGGAACTGCAGACAGATGAATTTGCCCAAGCGGCGGCAAGGGCGGCAATCCGTTTTTTGGAACGCTATGCGGGTTCGGG
CAACGAAGTGCTTGCAAACTGTACCGAACGCCTGTTTCAGGCATTGCAAAACGGTCATTCGTTTATCCGTTTGAGCGGGG
ACGAGGCTGACGCGCTGTCGGCACTCGCACCCGTGGTCGGAACATCCGCCGCGCCTTTGATTTTGGAAGGCAGAAGGCTG
TTTTTGGGCAGGATGTGGCAGTTGGAATACGATTTGGCTGCCGAAATAAAACGCTTGGCGGCAGCCGGCACGTCCGCCCC
CGACGCGGCAGGCGCGCGGCAAAACCTCGCAAAATGGTTTCAAGGCGCGGGAAGCGAAGGACAGCGCGATGCGGCCGCCT
TGGCACTGTTGCAGTTTTTTATGGTGATTACCGGAGGGCCGGGAACGGGCAAAACGACAACGGTTGCCAAACTGCTGGCA
CTGATTTGCGGTGAAAACGAAAATCTTCCCCATATCGCGCTTGCCGCACCGACGGGCAAAGCGGCGGCACATATGGCGCG
CGCACTGCACCGTTCAATCAACGGTTTTGACGCGCCGGAGGCCGTCCGCCGCCATTTGCTCAAACTGGAAGGGCAAACCG
TCCACCGACTGCTTAAGCTGAGCCCGCCCAAAATGCAGGCGGCGTTCGACCATATCCGTCCGCTGCCGTTCGACGTGCTG
ATTGTCGATGAAGCCTCCATGTTGGATACCGCATTGATGCTGCAACTTTTGAAAGCGGTCAAAACCGGCGCGCGCGTGAT
TCTGCTGGGCGATGAAAACCAGCTCCCGTCCGTCGGAATCGGGGCGGTGCTGTCCGTTTTGTCGCAAAAAACCGTTTTGG
ACGGAGAAACGCACCAAAGGCTGGCCGGTTTCCTTCCGGAACACGGTTTCAGCGTCAGCGCAAATCCGCCCGTGTTGGCG
CAAAACACCGCCCATCTGTCGTTCAGCCACCGCTTCGGCGACAACAGCGGCATCGGCTGCCTTGCCCGTGCCGCCGTATC
GGGCGATGAAGGGGCGTGGGCATTGTTTGACCGGTTTCCGGACGAACTGGAACATTCGGAATGCAGTCCGAATGCTCGGG
TCGAAAGGTTGTACCGGGCGCACAAAGCCTATTGGCAGGCGGTAAAAGACGGCAATATCGAAGCCGCATACGCAGGCATC
TCGGATATCGTGGTTCTGGCAGCTTGGCGGCAGGATGCGGAAGACTTCAACGAAGCCTATTGCAGCTATGTACGCCGCAA
AATGAACATACCGGAACATTTGGCCTATTTCGCCGGAGAGCCGATTATGATCAGACAGAACGACTACGCGCTTGAGCTGT
TCAACGGCGACATCGGACTGATTATGGAAGATGTCGGACGGCAGGGCAGCCTTGCCGCCTATTTTGCCGATGCGGACGGA
TTTAAAAAGGTAGCGGTAAGCTGCCTGCCCGAATTTGAACCCGCATTCGCCATGACCGTCCACAAAAGCCAAGGTTCGGA
ATATCGGGAAGTCTGGCTGCTGCCGCCTTCCGACGCACCTTCGGACGAAGGGGACGATGCATTGTCCGGATTGAGTAAGG
AGCTGTTATATACCGCCATTACCCGCGCCAGGGAAAAATTCGTATTTTTCGGCGGCAAAAAGACATTTTGTCAGGCGGTA
AACACCGTCAAAGTCCGTCAGACGGCGTTGGGCAGTATGCTTGAGCGGGTATTTTCACAAGAATAA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C_2) (494-548)

• AAA domain (AAA_30) (125-290)

3D structure

• AlphaFold DB: Q5F8K1

References

• [1]: I J Mehr et al. (1998) Differential roles of homologous recombination pathways in Neisseria gonorrhoeae pilin antigenic variation, DNA transformation and DNA repair. Molecular Microbiology 30(4):697-710. [PMID: 10094619]